Reduced erythrocyte deformability associated with hypoargininemia during Plasmodium falciparum malaria.
Reduced erythrocyte deformability associated with hypoargininemia during Plasmodium falciparum malaria.
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DOI：
10.1038/srep03767
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发表时间：
2014-01-20
影响因子：
4.6
通讯作者：
Safeukui I
中科院分区：
文献类型：
--
作者：
Rey J;Buffet PA;Ciceron L;Milon G;Mercereau-Puijalon O;Safeukui I
恶性疟原虫（Pf）疟疾期间红细胞（RBC）变形性降低的机制仍然知之甚少。在这里，我们探讨了l -精氨酸和一氧化氮（NO）途径在pf感染患者和寄生虫培养物中红细胞变形能力的可能参与。红细胞变形能力在急性发作（第0天）时降低，在恢复期（第28天）恢复正常。Day0值与血浆l -精氨酸水平相关（r = 0.69, p = 0.01），与寄生虫血症相关性较弱（r = - 0.38, p = 0.006）。体外，第0天患者血浆在41°C环状培养物中培养可降低红细胞变形能力，且这种作用与血浆l -精氨酸水平密切相关（r = 0.89; p < 0.0001）。此外，在培养物中添加外源l -精氨酸增加了不含pf和含有滋养体的红细胞的变形能力。NO合成酶活性，在nf感染的红细胞中得到证实，诱导l -精氨酸依赖的NO生成。这些数据表明，恶性疟原虫疟疾期间的低精氨酸血症可能完全损害NO的产生并降低红细胞的变形能力，特别是在发热温度下。
The mechanisms underlying reduced red blood cell (RBC) deformability during Plasmodium falciparum (Pf) malaria remain poorly understood. Here, we explore the possible involvement of the L-arginine and nitric oxide (NO) pathway on RBC deformability in Pf-infected patients and parasite cultures. RBC deformability was reduced during the acute attack (day0) and returned to normal values upon convalescence (day28). Day0 values correlated with plasma L-arginine levels (r = 0.69; p = 0.01) and weakly with parasitemia (r = −0.38; p = 0.006). In vitro, day0 patient's plasma incubated with ring-stage cultures at 41°C reduced RBC deformability, and this effect correlated strongly with plasma L-arginine levels (r = 0.89; p < 0.0001). Moreover, addition of exogenous L-arginine to the cultures increased deformability of both Pf-free and trophozoite-harboring RBCs. NO synthase activity, evidenced in Pf-infected RBCs, induced L-arginine-dependent NO production. These data show that hypoargininemia during P. falciparum malaria may altogether impair NO production and reduce RBC deformability, particularly at febrile temperature.
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影响因子：
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作者：
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通讯作者：
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