Functional properties of dopamine neurons and co-expression of vasoactive intestinal polypeptide in the dorsal raphe nucleus and ventro-lateral periaqueductal grey.
Functional properties of dopamine neurons and co-expression of vasoactive intestinal polypeptide in the dorsal raphe nucleus and ventro-lateral periaqueductal grey.
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DOI：
10.1111/j.1460-9568.2012.08255.x
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发表时间：
2012-11
期刊：
影响因子：
--
通讯作者：
Ungless MA
中科院分区：
文献类型：
--
作者：
Dougalis AG;Matthews GAC;Bishop MW;Brischoux F;Kobayashi K;Ungless MA
中缝背核(DRN)和中脑导水管周围灰质腹外侧区(VlPAG)含有多巴胺神经元群，通常被认为是A10群的尾部背侧延伸[主要发现于腹侧被盖区(VTA)]。最近的研究表明，它们参与促进觉醒，并调节鸦片类药物的一些抗伤害性和奖赏特性。然而，人们对它们的电生理特性知之甚少。为了解决这个问题，我们使用了Pitx3-GFP和酪氨酸羟基酶(TH)-GFP小鼠，在急性脑片中对这一群体进行了有针对性的全细胞记录。我们发现DRN/vlPAG多巴胺神经元具有与大多数VTA多巴胺神经元相似的特征，但不同于中缝背侧5-羟色胺神经元。它们以相对缓慢、规则的速度发出宽广的动作电位，表现出超极化激活的内向电流和延迟的复极，并对延长的去极化做出尖峰频率适应。此外，它们还接受快速兴奋性和抑制性突触输入。此外，我们发现血管活性肠多肽在中脑导水管周围的多巴胺神经元中共同表达，但通常在更大、更多的腹侧多巴胺神经元中不表达。
The dorsal raphe nucleus (DRN) and ventrolateral periaqueductal grey (vlPAG) regions contain populations of dopamine neurons, often considered to be a dorsal caudal extension of the A10 group [mostly found in the ventral tegmental area (VTA)]. Recent studies suggest they are involved in promoting wakefulness and mediate some of the antinociceptive and rewarding properties of opiates. However, little is known about their electrophysiological properties. To address this, we used Pitx3-GFP and tyrosine hydroxylase (TH)-GFP mice to carry out targeted whole-cell recordings from this population in acute brain slices. We found that DRN/vlPAG dopamine neurons have characteristics similar to most VTA dopamine neurons, but distinct from dorsal raphe serotonin neurons. They fire broad action potentials at a relatively slow, regular rate, exhibit a hyperpolarization-activated inward current and delayed repolarization, and show spike-frequency adaptation in response to prolonged depolarization. In addition, they receive fast excitatory and inhibitory synaptic inputs. Moreover, we found co-expression of vasoactive intestinal polypeptide in small, periaqueductal dopamine neurons, but generally not in larger, more ventral dopamine neurons.
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影响因子：
7.6
作者：
Flores, Juan A.;Galan-Rodriguez, Beatriz;Fernandez-Espejo, Emilio
通讯作者：
Fernandez-Espejo, Emilio
影响因子：
16.2
作者：
Lammel, Stephan;Hetzel, Andrea;Roeper, Jochen
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Roeper, Jochen
影响因子：
5.5
作者：
JOHNSON, SW;NORTH, RA
通讯作者：
NORTH, RA
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2.9
作者：
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通讯作者：
Arimura, A
影响因子：
15.8
作者：
Luescher, Christian;Ungless, Mark A.
通讯作者：
Ungless, Mark A.