Role of IL-24 in NK cell activation and its clinical implication in systemic lupus erythematosus
Role of IL-24 in NK cell activation and its clinical implication in systemic lupus erythematosus
复制标题
IL-24在系统性红斑狼疮NK细胞活化中的作用及其临床意义
DOI：
10.1007/s10067-021-05618-6
复制
发表时间：
2021-02
影响因子：
3.4
通讯作者：
Jianping Guo
中科院分区：
文献类型：
--
作者：
Yundi Tang;Xiaotong Sun;Yuxuan Wang;Huijie Luan;Ruijun Zhang;Fanlei Hu;Xiaolin Sun;Xia Li;Jianping Guo
目的白细胞介素（IL）-24被认为是自身免疫性疾病中的一种炎性细胞因子。然而，存在相互矛盾的数据，其生物学功能仍然存在争议。此外，人们对其对自然杀伤（NK）细胞的功能影响知之甚少。本研究旨在探讨IL-24在系统性红斑狼疮（SLE）患者NK细胞活化中的作用及其临床意义。方法检测299例SLE患者、214例RA患者和159例健康对照者血清中IL-24浓度，同时检测70例SLE患者、82例RA患者和123例健康对照者血浆中IL-24浓度。在两个NK细胞亚群中评估IL-24对NK细胞活化的影响，即，CD56dimCD16+和CD56brightCD16 − NK细胞。应用人NK-92细胞系评价IL-24对NK细胞迁移和侵袭的功能潜力。结果SLE、RA和HC患者血清和血浆IL-24水平相当。虽然重组人（rh）IL-2始终诱导来自健康受试者和SLE患者的CD56dimCD16+和CD56brightCD16 −细胞上的CD69表达增加，但单独的IL-24不足以激活CD56dimNK细胞和CD56brightNK细胞。同样，虽然迁移NK-92细胞的数量显着增加与rhIL-2的刺激，IL-24单独是无法提高NK-92细胞的迁移和侵袭capability.ConclusionOur数据表明，SLE患者和健康对照之间的血清和血浆中的IL-24浓度没有显着差异。重组IL-24对NK细胞活化和迁移没有影响。关键点·这是首次研究IL-24对NK细胞活化的功能潜力。·重组IL-24缺乏对来自健康受试者和SLE患者的CD56dimCD16+或CD56brightCD16-NK细胞亚群中NK细胞活化的功能能力。SLE患者血清和血浆IL-24水平与健康对照组无显著差异。
ObjectivesInterleukin (IL)-24 has been considered as an inflammatory cytokine in autoimmune diseases. However, conflicting data exist and its biological function remains controversial. Additionally, little is known about its functional impact on natural killer (NK) cells. The aim of this study was to investigate the role of IL-24 in NK cell activation and its clinical implication in systemic lupus erythematosus (SLE).MethodsSerum cohort consisting of 299 SLE patients, 214 RA patients, and 159 healthy controls (HCs) and plasma cohort consisting of 70 SLE patients, 82 RA patients, and 123 HCs were included in evaluating IL-24 concentrations. Impact of IL-24 on NK cell activation was assessed in two NK cell subsets, i.e., CD56dimCD16+and CD56brightCD16−NK cells. Human NK-92 cell line was applied to evaluate functional potential of IL-24 on NK cell migration and invasion.ResultsSerum and plasma levels of IL-24 were comparable between patients with SLE or RA and HCs. While recombinant human (rh) IL-2 consistently induced an increased expression of CD69 on both CD56dimCD16+and CD56brightCD16−cells derived from both healthy subjects and patients with SLE, IL-24 alone was insufficient to activate the CD56dimand CD56brightNK cells. Similarly, while the migratory NK-92 cell numbers were significantly increased with rhIL-2 stimulation, IL-24 alone was unable to enhance NK-92 cell migratory and invasive capacity.ConclusionOur data indicate that there were no significant differences in serum and plasma concentrations of IL-24 between SLE patients and healthy controls. Recombinant IL-24 has no effect on NK cell activation and migration.Key points•This is the first study to investigate functional potential of IL-24 on NK cell activation.•Recombinant IL-24 lacks functional capacity on NK cell activation in either CD56dimCD16+or CD56brightCD16-NK cell subsets derived from both healthy subjects and patients with SLE.•No significant differences in serum and plasma levels of IL-24 between SLE patients and healthy controls.
登录
查看更多内容
DOI：
10.1073/pnas.152327199
发表时间：
2002-07-23
影响因子：
11.1
作者：
Sarkar, D;Su, ZZ;Fisher, PB
通讯作者：
Fisher, PB
影响因子：
2.6
作者：
Li, R. C.;Guo, J.;Huang, A. F.
通讯作者：
Huang, A. F.
影响因子：
4.4
作者：
A. Nagler;L. Lanier;S. Cwirla;J. Phillips
通讯作者：
A. Nagler;L. Lanier;S. Cwirla;J. Phillips
影响因子：
4.4
作者：
Caudell, EG;Mumm, JB;Grimm, EA
通讯作者：
Grimm, EA
影响因子：
3.8
作者：
Kragstrup, Tue Wenzel;Otkjaer, Kristian;Deleuran, Bent
通讯作者：
Deleuran, Bent