Selected activities of Citrus maxima Merr. fruits on human endothelial cells: enhancing cell migration and delaying cellular aging.-猫眼课题宝

DOI：

10.3390/nu6041618

发表时间：

2014-04-21

期刊：

Nutrients

影响因子：

5.9

通讯作者：

中科院分区：

医学2区

文献类型：

作者：

Buachan P;Chularojmontri L;Wattanapitayakul SK

来源链接：

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4011055/?report=classic

衰老过程中内皮损伤和损伤以及活性氧（ROS）的积累在心血管疾病（CVD）的发展中发挥着重要作用。最近的研究表明，大量摄入柑橘类水果与降低心血管疾病和中风风险之间存在关联，但其中的机制尚不完全清楚。本研究调查了柚子 (Citrus maxima Merr. var. Tubtim Siam, CM) 果实提取物对人脐静脉内皮细胞 (HUVEC) 迁移和衰老的影响。对水果提取物冻干粉的抗氧化能力（FRAP 测定）和某些天然抗氧化剂进行了表征，包括抗坏血酸、没食子酸、橙皮苷和柚皮苷（HPLC）。通过划伤试验和博伊登室试验评估，HUVEC 与 CM 的短期（48 小时）共培养增强了细胞迁移。群体倍增水平 (PDL) 表明，35 天的 CM 长期治疗显着提高了 HUVEC 增殖能力。 CM 还延迟了衰老相关 β-半乳糖苷酶 (SA-β-gal) 染色显示的衰老表型的发生。此外，当通过 2',7'-二氯二氢荧光素二乙酸酯 (DCDHF) 测定时，CM 能够减弱衰老细胞中增加的 ROS 水平，同时 eNOS mRNA 表达增加，但 eNOS 蛋白水平没有改变。因此，需要进一步的体内和临床研究来支持柚子作为内皮健康和降低心血管疾病风险的功能性水果的使用。

Endothelial injury and damage as well as accumulated reactive oxygen species (ROS) in aging play a significant role in the development of cardiovascular disease (CVD). Recent studies show an association of high citrus fruit intake with a lower risk of CVD and stroke but the mechanisms involved are not fully understood. This study investigated the effects of pummelo (Citrus maxima Merr. var. Tubtim Siam, CM) fruit extract on human umbilical vein endothelial cell (HUVECs) migration and aging. The freeze-dried powder of fruit extract was characterized for antioxidant capacity (FRAP assay) and certain natural antioxidants, including ascorbic acid, gallic acid, hesperidin, and naringin (HPLC). Short-term (48 h) co-cultivation of HUVECs with CM enhanced cell migration as evaluated by a scratch wound assay and Boyden chamber assay. A long-term treatment with CM for 35 days significantly increased HUVEC proliferation capability as indicated by population doubling level (PDL). CM also delayed the onset of aging phenotype shown by senescence-associated β-galactosidase (SA-β-gal) staining. Furthermore, CM was able to attenuate increased ROS levels in aged cells when determined by 2′,7′-dichlorodihydrofluorescein diacetate (DCDHF) while eNOS mRNA expression was increased but the eNOS protein level was not changed. Thus, further in vivo and clinical studies are warranted to support the use of pummelo as a functional fruit for endothelial health and CVD risk reduction.