The three NADH dehydrogenases of Pseudomonas aeruginosa: Their roles in energy metabolism and links to virulence.
The three NADH dehydrogenases of Pseudomonas aeruginosa: Their roles in energy metabolism and links to virulence.
复制标题
铜绿假单胞菌的三种NADH脱氢酶：它们在能量代谢中的作用以及与毒力的联系。
DOI：
10.1371/journal.pone.0244142
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Barquera B
中科院分区：
文献类型：
--
作者：
Hreha TN;Foreman S;Duran-Pinedo A;Morris AR;Diaz-Rodriguez P;Jones JA;Ferrara K;Bourges A;Rodriguez L;Koffas MAG;Hahn M;Hauser AR;Barquera B
铜绿假单胞菌是一种普遍存在的条件致病菌，它依靠高度适应性的代谢来实现广泛的发病机制。在这种灵活性的一个例子中，为了催化呼吸链的NADH：醌氧化还原酶步骤，P. aeruginosa有三种不同的酶：NUO， NQR和NDH2，它们都具有相同的氧化还原功能，但具有不同的能量节约和离子运输特性。为了更好地理解这些酶的作用，我们构建了两个系列的突变体：(i)三个单缺失突变体，每个缺失一个NADH脱氢酶；（ii）三个双缺失突变体，每个只保留三种酶中的一种。当在丰富和最小的培养基以及pH和[Na+]条件范围内进行测试时，所有突变体的生长都与野生型大致相同，除了仅含有NUO的菌株（ΔnqrFΔndh）具有延长的滞后期。在指数期，NADH脱氢酶对野生型总活性的贡献顺序为：NQR > NDH2 > NUO。一些突变体，包括没有NQR的菌株（ΔnqrF），在巨噬细胞和小鼠感染模型中都增加了生物膜的形成和pyocyanin的产生，并且更有效地杀死了它们。与此一致的是，ΔnqrF显示与花青素生产有关的基因转录增加。
Pseudomonas aeruginosa is a ubiquitous opportunistic pathogen which relies on a highly adaptable metabolism to achieve broad pathogenesis. In one example of this flexibility, to catalyze the NADH:quinone oxidoreductase step of the respiratory chain, P. aeruginosa has three different enzymes: NUO, NQR and NDH2, all of which carry out the same redox function but have different energy conservation and ion transport properties. In order to better understand the roles of these enzymes, we constructed two series of mutants: (i) three single deletion mutants, each of which lacks one NADH dehydrogenase and (ii) three double deletion mutants, each of which retains only one of the three enzymes. All of the mutants grew approximately as well as wild type, when tested in rich and minimal medium and in a range of pH and [Na+] conditions, except that the strain with only NUO (ΔnqrFΔndh) has an extended lag phase. During exponential phase, the NADH dehydrogenases contribute to total wild-type activity in the following order: NQR > NDH2 > NUO. Some mutants, including the strain without NQR (ΔnqrF) had increased biofilm formation, pyocyanin production, and killed more efficiently in both macrophage and mouse infection models. Consistent with this, ΔnqrF showed increased transcription of genes involved in pyocyanin production.
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影响因子：
14.8
作者：
Hmelo LR;Borlee BR;Almblad H;Love ME;Randall TE;Tseng BS;Lin C;Irie Y;Storek KM;Yang JJ;Siehnel RJ;Howell PL;Singh PK;Tolker-Nielsen T;Parsek MR;Schweizer HP;Harrison JJ
通讯作者：
Harrison JJ
影响因子：
5.2
作者：
Arai H
通讯作者：
Arai H
影响因子：
4.4
作者：
Allen, L;Dockrell, DH;Whyte, MKB
通讯作者：
Whyte, MKB
影响因子：
4.2
作者：
Fothergill JL;Panagea S;Hart CA;Walshaw MJ;Pitt TL;Winstanley C
通讯作者：
Winstanley C
影响因子：
2.9
作者：
Barquera, B;Nilges, MJ;Gennis, RB
通讯作者：
Gennis, RB