Widespread pyocyanin over-production among isolates of a cystic fibrosis epidemic strain.
Widespread pyocyanin over-production among isolates of a cystic fibrosis epidemic strain.
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DOI：
10.1186/1471-2180-7-45
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发表时间：
2007-05-23
期刊：
影响因子：
4.2
通讯作者：
Winstanley C
中科院分区：
文献类型：
--
作者：
Fothergill JL;Panagea S;Hart CA;Walshaw MJ;Pitt TL;Winstanley C
铜绿假单胞菌的利物浦囊性纤维化流行株的一些分离株表现出不寻常的毒力相关表型，其特征在于过量产生群体感应调节的外产物，如绿脓菌素和LasA蛋白酶。我们的目的是确定流行株分离株之间这种不寻常的表型的患病率，并研究其他克隆内表型和基因型变异。在大多数接受测试的囊性纤维化患者中，至少有一种流行株分离株中检测到了这种不寻常的表型，并且在慢性感染期间可以保留长达7年。在长达9年的时间内，从6名患者身上采集的流行菌株的多个连续分离株表现出广泛的表型，包括不同的抗菌药物敏感性。我们的数据表明，每个痰液样本包含流行菌株群体内的表型和基因型的混合物，包括菌落形态型。许多分离株表现出过早（在早期而不是晚期指数生长期间）和过度生产绿脓菌素，其具有许多与囊性纤维化直接相关的毒性作用。这种不寻常的表型的广泛发生表明，它可能在流行株的成功中发挥重要作用。
Some isolates of the Liverpool cystic fibrosis epidemic strain of Pseudomonas aeruginosa exhibit an unusual virulence-related phenotype, characterized by over-production of quorum sensing-regulated exoproducts such as pyocyanin and LasA protease. Our aim was to determine the prevalence of this unusual phenotype amongst isolates of the epidemic strain, and to study other intraclonal phenotypic and genotypic variations. The unusual phenotype was detected in at least one epidemic strain isolate from the majority of cystic fibrosis patients tested, and can be retained for up to seven years during chronic infection. Multiple sequential isolates of the epidemic strain taken from six patients over a period of up to nine years exhibited a wide range of phenotypes, including different antimicrobial susceptibilities. Our data suggest that each sputum sample contains a mixture of phenotypes and genotypes within the epidemic strain population, including within colony morphotypes. Many isolates exhibit premature (during early rather than late exponential growth) and over-production of pyocyanin, which has a number of toxic effects directly relevant to cystic fibrosis. The widespread occurrence of this unusual phenotype suggests that it may play an important role in the success of the epidemic strain.
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影响因子：
5.2
作者：
Andrews, JM
通讯作者：
Andrews, JM
影响因子：
9.4
作者：
Parsons, YN;Panagea, S;Winstanley, C
通讯作者：
Winstanley, C
影响因子：
10
作者：
Al-Aloul, M;Crawley, J;Walshaw, MJ
通讯作者：
Walshaw, MJ
DOI：
10.1152/ajplung.00086.2003
发表时间：
2003-09-01
影响因子：
4.9
作者：
Denning, GM;Iyer, SS;Britigan, BE
通讯作者：
Britigan, BE
影响因子：
4.4
作者：
Allen, L;Dockrell, DH;Whyte, MKB
通讯作者：
Whyte, MKB