Engineered Tissue Folding by Mechanical Compaction of the Mesenchyme.
Engineered Tissue Folding by Mechanical Compaction of the Mesenchyme.
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DOI：
10.1016/j.devcel.2017.12.004
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发表时间：
2018-01-22
影响因子：
11.8
通讯作者：
Gartner ZJ
中科院分区：
文献类型：
--
作者：
Hughes AJ;Miyazaki H;Coyle MC;Zhang J;Laurie MT;Chu D;Vavrušová Z;Schneider RA;Klein OD;Gartner ZJ
许多组织在发育过程中折叠成复杂的形状。在体外控制这一过程将代表组织工程的一个重要进展。我们使用胚胎组织外植体、有限元建模和3D细胞图案化技术来显示间充质凝聚期间ECM的机械压实足以驱动组织沿沿着编程轨迹折叠。该过程需要细胞收缩性，在组织界面产生应变，并导致冷凝物周围和之间的胶原蛋白排列模式。排列的胶原纤维支持升高的张力，其促进界面沿沿着路径折叠，所述路径可通过建模来预测。我们展示了这种策略的鲁棒性和多功能性，通过指导各种折叠组织形式的间充质凝聚物的模式的形态发生来雕刻组织界面。这些研究提供了对胚胎间充质的主动机械特性的深入了解，并建立了更有力地指导离体组织形态发生的工程策略。组织工程的一个关键挑战是利用体内的组织原理来产生复杂的折叠界面。Hughes等人发现，间充质压实过程足以在组织界面处产生曲率，并开发了一种框架，以通过间充质凝聚物的模式来设计重建组织的曲率轨迹。
Many tissues fold into complex shapes during development. Controlling this process in vitro would represent an important advance for tissue engineering. We use embryonic tissue explants, finite element modeling, and 3D cell patterning techniques to show that mechanical compaction of the ECM during mesenchymal condensation is sufficient to drive tissue folding along programmed trajectories. The process requires cell contractility, generates strains at tissue interfaces, and causes patterns of collagen alignment around and between condensates. Aligned collagen fibers support elevated tensions that promote the folding of interfaces along paths that can be predicted by modeling. We demonstrate the robustness and versatility of this strategy for sculpting tissue interfaces by directing the morphogenesis of a variety of folded tissue forms from patterns of mesenchymal condensates. These studies provide insight into the active mechanical properties of the embryonic mesenchyme and establish engineering strategies for more robustly directing tissue morphogenesis ex vivo. A key challenge for tissue engineering is harnessing organizational principles operating in vivo to generate complex folded interfaces. Hughes et al. find that mesenchymal compaction processes are sufficient to generate curvature at tissue interfaces and develop a framework to engineer curvature trajectories of reconstituted tissues via patterns of mesenchymal condensates.
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影响因子：
13.6
作者：
Hinton TJ;Jallerat Q;Palchesko RN;Park JH;Grodzicki MS;Shue HJ;Ramadan MH;Hudson AR;Feinberg AW
通讯作者：
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作者：
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通讯作者：
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