Hospital and community ampicillin-resistant Enterococcus faecium are evolutionarily closely linked but have diversified through niche adaptation.
Hospital and community ampicillin-resistant Enterococcus faecium are evolutionarily closely linked but have diversified through niche adaptation.
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DOI：
10.1371/journal.pone.0030319
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Willems RJ
中科院分区：
文献类型：
--
作者：
de Regt MJ;van Schaik W;van Luit-Asbroek M;Dekker HA;van Duijkeren E;Koning CJ;Bonten MJ;Willems RJ
耐氨苄青霉素屎肠球菌 (ARE) 已成为一种医院病原体。在这里，我们量化了不同社区来源中的 ARE 携带情况，并确定了与医院 ARE 的遗传相关性。 ARE 是从 79 只狗中的 24 只 (30%)、85 只猫中的 11 只 (13%) 和 42 匹马中的 0 只的直肠拭子中以及从接受阿莫西林的 40 名非住院患者的粪便样本中的 3 份 (8%) 中回收的。多位点序列分型揭示了 21 种序列类型 (ST)，其中包括 5 种经常与医院获得性感染相关的 ST。先前发现在医院 ARE 中富集的基因，如 IS16、orf903、orf905、orf907，在社区 ARE 中非常普遍（≥79%），而在发病机制中发挥作用的基因，如 esp、hyl 和 ecbA，在社区分离株中很少发现（≤5%）。对 2 个代表性犬株的比较基因组分析表明，ST192 犬品系在进化上与之前测序的两个医院 ARE 密切相关，但根据基因内容，与另一个进化上关系较远的犬品系 (ST266) 有更多共同基因。在狗、猫和健康人类中都检测到了 ARE，这与医院的 ARE 存在进化联系。然而，它们的附属基因组已经多样化，这可能是生态位适应的结果。
Ampicillin-resistant Enterococcus faecium (ARE) has emerged as a nosocomial pathogen. Here, we quantified ARE carriage in different community sources and determined genetic relatedness with hospital ARE. ARE was recovered from rectal swabs of 24 of 79 (30%) dogs, 11 of 85 (13%) cats and 0 of 42 horses and from 3 of 40 (8%) faecal samples of non-hospitalized humans receiving amoxicillin. Multi-locus Sequence Typing revealed 21 sequence types (STs), including 5 STs frequently associated with hospital-acquired infections. Genes previously found to be enriched in hospital ARE, such as IS16, orf903, orf905, orf907, were highly prevalent in community ARE (≥79%), while genes with a proposed role in pathogenesis, such as esp, hyl and ecbA, were found rarely (≤5%) in community isolates. Comparative genome analysis of 2 representative dog isolates revealed that the dog strain of ST192 was evolutionarily closely linked to two previously sequenced hospital ARE, but had, based on gene content, more genes in common with the other, evolutionarily more distantly related, dog strain (ST266). ARE were detected in dogs, cats and sporadically in healthy humans, with evolutionary linkage to hospital ARE. Yet, their accessory genome has diversified, probably as a result of niche adaptation.
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影响因子：
5.2
作者：
Khan, Mushtaq A.;van der Wal, Martin;Hays, John P.
通讯作者：
Hays, John P.
DOI：
10.1126/science.1182395
发表时间：
2010-01-22
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Harris SR;Feil EJ;Holden MT;Quail MA;Nickerson EK;Chantratita N;Gardete S;Tavares A;Day N;Lindsay JA;Edgeworth JD;de Lencastre H;Parkhill J;Peacock SJ;Bentley SD
通讯作者：
Bentley SD
影响因子：
3.2
作者：
Heikens, Esther;Bonten, Marc J. M.;Willems, Rob J. L.
通讯作者：
Willems, Rob J. L.
影响因子：
3.3
作者：
Boerlin, P;Eugster, S;Schawalder, P
通讯作者：
Schawalder, P
影响因子：
9.4
作者：
Homan, WL;Tribe, D;Willems, RJL
通讯作者：
Willems, RJL