Influence of the oxygen microenvironment on the proangiogenic potential of human endothelial colony forming cells.
Influence of the oxygen microenvironment on the proangiogenic potential of human endothelial colony forming cells.
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DOI：
10.1007/s10456-009-9152-6
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发表时间：
2009
期刊：
影响因子：
9.8
通讯作者：
Leach, J. Kent
中科院分区：
文献类型：
--
作者：
Decaris, Martin L.;Lee, Chang I.;Yoder, Mervin C.;Tarantal, Alice F.;Leach, J. Kent
治疗性血管生成是一种很有前景的策略，可以促进新血管或侧支血管的形成，用于组织的再生和修复。由于已知组织氧浓度的变化会刺激多种细胞功能，这些研究主要集中在氧微环境及其对内皮细胞血管生成潜能的作用。我们分析了人内皮细胞集落形成细胞(HECFCs)的前血管生成潜力，hECFCs是一种高度增殖的循环内皮祖细胞群体，并比较了在氧分压从1%到21%O2的范围内，代表缺血或健康组织和标准培养条件的人真皮微血管细胞(HMVECs)的结果。与HMVECs相比，hECFCs(1)在缺血条件和环境空气中表现出明显更强的增殖；(2)与HMVECs相比，当暴露于低氧的趋化梯度时，hECFC表现出更多的迁移；(3)当使用血管形成实验评估时，hECFC在低氧条件下表现出类似或更好的促血管生成潜力。这些数据表明，两种内皮细胞的血管生成潜力都受到局部氧气微环境的影响。然而，hECFC在代表生理性、缺血性或环境空气条件的氧气条件下表现出强大的血管生成潜力，这些发现表明hECFC可能是用于缺血或工程化组织新生血管的基于细胞的方法的优良细胞来源。
Therapeutic angiogenesis is a promising strategy to promote the formation of new or collateral vessels for tissue regeneration and repair. Since changes in tissue oxygen concentrations are known to stimulate numerous cell functions, these studies have focused on the oxygen microenvironment and its role on the angiogenic potential of endothelial cells. We analyzed the proangiogenic potential of human endothelial colony-forming cells (hECFCs), a highly proliferative population of circulating endothelial progenitor cells, and compared outcomes to human dermal microvascular cells (HMVECs) under oxygen tensions ranging from 1% to 21% O2, representative of ischemic or healthy tissues and standard culture conditions. Compared to HMVECs, hECFCs (1) exhibited significantly greater proliferation in both ischemic conditions and ambient air; (2) demonstrated increased migration compared to HMVECs when exposed to chemotactic gradients in reduced oxygen; and (3) exhibited comparable or superior proangiogenic potential in reduced oxygen conditions when assessed using a vessel-forming assay. These data demonstrate that the angiogenic potential of both endothelial populations is influenced by the local oxygen microenvironment. However, hECFCs exhibit a robust angiogenic potential in oxygen conditions representative of physiologic, ischemic, or ambient air conditions, and these findings suggest that hECFCs may be a superior cell source for use in cell-based approaches for the neovascularization of ischemic or engineered tissues.
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DOI：
10.1002/jbm.10134
发表时间：
2002-06-15
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
Peters, MC;Polverini, PJ;Mooney, DJ
通讯作者：
Mooney, DJ
影响因子：
4.8
作者：
Namiki, A;Brogi, E;Isner, JM
通讯作者：
Isner, JM
影响因子：
9.8
作者：
Donovan, D.;Brown, N. J.;Lewis, C. E.
通讯作者：
Lewis, C. E.
影响因子：
20.1
作者：
O'Neill, TJ;Wamhoff, BR;Skalak, TC
通讯作者：
Skalak, TC
影响因子：
3.7
作者：
Leu, Ann;Leach, J. Kent
通讯作者：
Leach, J. Kent