Measurement of the distribution of aerosols among mouse lobes by fluorescent imaging.
Measurement of the distribution of aerosols among mouse lobes by fluorescent imaging.
复制标题
通过荧光成像测量小鼠肺叶中气溶胶的分布。
DOI：
10.1016/j.ab.2010.04.005
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发表时间：
2010
影响因子：
2.9
通讯作者：
Wiedmann,TimothyScott
中科院分区：
文献类型：
--
作者：
Yi,Dandan;Price,Andrew;Panoskaltsis-Mortari,Angela;Naqwi,Amir;Wiedmann,TimothyScott
制备肺组织标本，研究光吸收对四磺酸铝(AlPCS)荧光信号定量的扰动效应。将已知浓度和深度的标准溶液在不同曝光时间下成像并进行分析。强度与浓度、深度、曝光时间和面积呈线性函数关系。小鼠暴露于AlPCS气雾剂中，其质量中值空气动力学直径为390 nm，几何标准偏差为1.8。获得完整的肺叶和肺匀浆的图像，然后进行分析，以量化肺叶和气管中的AlPCS浓度。对于气溶胶的分布，结果表明，气溶胶的浓度在不同的叶之间是均匀的。结合浓度的定量分析和面积/厚度的图像分析，很容易确定每个叶中沉积的质量。该方法为确定药物对小鼠下呼吸道的选择性提供了一种定量手段。
Lung samples were prepared to investigate the perturbing effects of light absorption for quantifying the fluorescence signal of aluminum phthalocyanine tetrasulfonic acid (AlPCS). Standard solutions of known concentration and depth were imaged with different exposure times and analyzed. The intensity was found to be a linear function of concentration, depth, exposure time, and area. Mice were exposed to an aerosol of AlPCS with a mass median aerodynamic diameter of 390nm and geometric standard deviation of 1.8. Images of intact lung lobes and lung homogenates were obtained and then analyzed to allow quantifying the concentration of AlPCS among the lung lobes and trachea. For the distribution of aerosols, the results indicate that the concentration was uniform among the different lobes. Combining the quantitative analysis of the concentration with image analysis of the area/thickness, the mass deposited in each lobe was readily determined. This approach provides a quantitative means to determine the selectivity of drug delivery to mouse lower respiratory tract.
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DOI：
10.1089/jam.2006.19.8
发表时间：
2006
期刊：
Journal of aerosol medicine : the official journal of the International Society for Aerosols in Medicine
影响因子：
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作者：
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作者：
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发表时间：
2008-12-01
影响因子：
3.4
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影响因子：
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作者：
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DOI：
10.1089/jamp.2008.0693
发表时间：
2009-06-01
影响因子：
3.4
作者：
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