Measurement of Hydroxyl-Radical Formation in the Rat Striatum by In Vivo Microdialysis and GC-MS.
Measurement of Hydroxyl-Radical Formation in the Rat Striatum by In Vivo Microdialysis and GC-MS.
复制标题
通过体内微透析和GC-MS测量大鼠纹状体中羟基形成。
DOI：
10.1365/s10337-008-0703-6
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发表时间：
2008-10
期刊：
影响因子：
1.7
通讯作者：
Prokai L
中科院分区：
文献类型：
--
作者：
Nguyen V;Bonds DV;Prokai L
建立了一种GC-MS法测定活体脑微透析实验样品中水杨酸的羟基自由基捕获产物。方法采用液-液萃取，2，3-和2，5-二羟基苯甲酸衍生化，以3，5-二羟基苯甲酸为内标。由于叔丁基二甲基硅基(TBDMS)衍生物通过失去相应分子离子中的叔烷基团而以[M-57]+离子为特征的简单电子电离质谱图，因此提供了基于选择离子监测的直接方法发展。此外，串联质谱仪探测了从叔丁基二甲基硅烷同分异构体获得的[M-57]+离子的碰撞诱导解离，结果显示生成的产物-离子光谱特征不同。我们的工作证明了GC-MS在2，3-和2，5-二羟基苯甲酸微透析液分析中的适用性，证实了局部向大鼠纹状体注射兴奋性毒性谷氨酸显著增加了体内该脑区羟基自由基的产生，随后全身注射α-苯基-叔丁基硝酮逆转了谷氨酸诱导的氧化应激。
A GC-MS method was developed for measuring hydroxyl-radical capture products of salicylic acid, a common trapping agent for this reactive oxygen species, in samples obtained by in vivo cerebral microdialysis experiments. The assay employed liquid-liquid extraction followed by derivatization of 2,3- and 2,5-dihydroxybenzoic acid, along with 3,5-dihydroxybenzoic acid added as an internal standard. Due to their simple electron ionization mass spectra featuring [M – 57]+ ions through the loss of tertiary alkyl group from the corresponding molecular ions, tert-butyldimethylsilyl (TBDMS) derivatives afforded straightforward method development based on selected-ion monitoring. In addition, tandem mass spectrometry probing collision-induced dissociation of [M – 57]+ ions obtained from the isomeric tert-butyldimethylsilyl derivatives revealed characteristic differences in the resultant product-ion spectra. Our work has demonstrated the applicability of GC-MS for the assay of microdialysates for 2,3- and 2,5-dihydroxybenzoic acid by confirming that local administration of the excitotoxic glutamate into the rat striatum significantly increased in vivo hydroxyl-radical production in this brain region and that subsequent systemic administration of α-phenyl-tert-butylnitrone reversed glutamate-induced oxidative stress.
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影响因子：
4.1
作者：
SWANSON, LW;SAWCHENKO, PE
通讯作者：
SAWCHENKO, PE
DOI：
10.1016/s1385-299x(00)00014-3
发表时间：
2000-04-01
期刊：
BRAIN RESEARCH PROTOCOLS
影响因子：
--
作者：
Teismann, P;Ferger, B
通讯作者：
Ferger, B
影响因子：
3
作者：
Themann, C;Teismann, P;Ferger, B
通讯作者：
Ferger, B
影响因子：
5.6
作者：
Wan, FJ;Tung, CS;Lin, HC
通讯作者：
Lin, HC
影响因子：
1.7
作者：
Thanikachalam, Sivakumar;Rajappan, Manavalan;Kannappan, Valliappan
通讯作者：
Kannappan, Valliappan