NanI Sialidase Contributes to the Growth and Adherence of Clostridium perfringens Type F Strain F4969 in the Presence of Adherent Mucus.
NanI Sialidase Contributes to the Growth and Adherence of Clostridium perfringens Type F Strain F4969 in the Presence of Adherent Mucus.
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NanI 唾液酸酶有助于产气荚膜梭菌 F 型菌株 F4969 在粘附粘液存在下的生长和粘附。
DOI：
10.1128/iai.00256-21
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发表时间：
2021
影响因子：
3.1
通讯作者：
McClane,BruceA
中科院分区：
文献类型：
--
作者：
Li,Jihong;Navarro,MauricioA;Uzal,FranciscoA;McClane,BruceA
产气荚膜梭菌F型菌株引起非食源性人类胃肠道疾病（NFD），其主要分泌唾液酸酶为NanI唾液酸酶。F型NFDs可持续数周，表明其发病机制涉及肠道定植，包括营养细胞的生长和粘附，随后的产孢促进肠毒素的产生和释放。我们之前报道过NanI有助于F型NFD菌株的粘附和Caco-2细胞的生长。然而，Caco-2细胞产生的黏液很少，这一点很重要，因为肠道被黏液覆盖。因此，评估NanI是否有助于F型NFD菌株在黏附粘液存在下的生长和粘附是很重要的。因此，目前的研究首次证明，在HT29- mtx - e12细胞存在的情况下，携带纳米颗粒的F型菌株比不携带纳米颗粒的F型菌株生长得更快，HT29- mtx - e12细胞产生黏液层，而它们的亲本HT29细胞产生黏液很少。在HT29- mtx - e12细胞和HT29细胞存在的情况下，F型NFD菌株F4969或互补菌株比等基因naninull突变株生长和粘附更好，证明了NanI对这种作用的特殊重要性。这些影响涉及HT29-MTX-E12细胞产生粘液，因为使用n -乙酰半胱氨酸减少粘液会减少F4969的生长和粘附。与体外实验结果一致，NanI促进了小鼠小肠中F4969的生长。通过证明NanI在黏附粘液存在下的生长和粘附作用，这些结果进一步支持NanI作为F型NFDs的潜在毒力因子。
Clostridium perfringens type F strains causing nonfoodborne human gastrointestinal diseases (NFD) typically produce NanI sialidase as their major secreted sialidase. Type F NFDs can persist for several weeks, indicating their pathogenesis involves intestinal colonization, including vegetative cell growth and adherence, with subsequent sporulation that fosters enterotoxin production and release. We previously reported that NanI contributes to type F NFD strain adherence and growth using Caco-2 cells. However, Caco-2 cells make minimal amounts of mucus, which is significant because the intestines are coated with adherent mucus. Therefore, it was important to assess if NanI contributes to the growth and adherence of type F NFD strains in the presence of adherent mucus. Consequently, the current study first demonstrated greater growth ofnanI-carrying versus non-nanI-carrying type F strains in the presence of HT29-MTX-E12 cells, which produce an adherent mucus layer, versus their parental HT29 cells, which make minimal mucus. Demonstrating the specific importance of NanI for this effect, type F NFD strain F4969 or a complementing strain grew and adhered better than an isogenicnanInull mutant in the presence of HT29-MTX-E12 cells versus HT29 cells. Those effects involved mucus production by HT29-MTX-E12 cells since mucus reduction usingN-acetyl cysteine reduced F4969 growth and adherence. Consistent with thosein vitroresults, NanI contributed to growth of F4969 in the mouse small intestine. By demonstrating a growth and adherence role for NanI in the presence of adherent mucus, these results further support NanI as a potential virulence factor during type F NFDs.
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DOI：
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