The RNA-RNA interactome between a phage and its satellite virus reveals a small RNA that differentially regulates gene expression across both genomes.
The RNA-RNA interactome between a phage and its satellite virus reveals a small RNA that differentially regulates gene expression across both genomes.
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DOI：
10.1111/mmi.15046
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发表时间：
2023-04
影响因子：
3.6
通讯作者：
Seed, Kimberley D.
中科院分区：
文献类型：
--
作者：
Dunham, Drew T.;Angermeyer, Angus;Seed, Kimberley D.
卫星病毒存在于生命的各个领域，被定义为需要另一种病毒感染才能产生卫星后代的亚病毒寄生虫。噬菌体卫星表现出各种调节机制来操纵噬菌体基因表达以使卫星受益，将资源从噬菌体重定向到卫星，并经常抑制噬菌体后代的产生。虽然小 RNA (sRNA) 作为原核基因表达的调节因子已被充分证明，但尚未证明它们在卫星噬菌体冲突中发挥调节作用。霍乱弧菌编码噬菌体诱导型染色体岛样元件 (PLE)（一种噬菌体卫星），以防御裂解噬菌体 ICP1。在这里，我们使用 Hi-GRIL-seq 来识别 PLE 和 ICP1 之间的复杂 RNA-RNA 相互作用组。检测到基因组间和基因组内 RNA 相互作用，以 PLE sRNA、SviR 为标题。 SviR 独特地参与调节 PLE 和 ICP1 基因表达，减少 ICP1 靶标翻译并影响 PLE 转录本。 SviR 在所有已知 PLE 中的惊人保守性表明 sRNA 深深植根于 PLE-ICP1 冲突，并暗示 sRNA 在噬菌体-卫星相互作用中是基因表达的未知调节因子。
Satellite viruses are present across all domains of life, defined as sub-viral parasites that require infection by another virus for satellite progeny production. Phage satellites exhibit various regulatory mechanisms to manipulate phage gene expression to the benefit of the satellite, redirecting resources from the phage to the satellite, and often inhibiting phage progeny production. While small RNAs (sRNAs) are well documented as regulators of prokaryotic gene expression, they have not been shown to play a regulatory role in satellite-phage conflicts. Vibrio cholerae encodes the phage inducible chromosomal island-like element (PLE), a phage satellite, to defend itself against the lytic phage ICP1. Here we use Hi-GRIL-seq to identify a complex RNA-RNA interactome between PLE and ICP1. Both inter- and intra-genome RNA interactions were detected, headlined by the PLE sRNA, SviR. SviR is involved in regulating both PLE and ICP1 gene expression uniquely, decreasing ICP1 target translation and affecting PLE transcripts. The striking conservation of SviR across all known PLEs suggests the sRNA is deeply rooted in the PLE-ICP1 conflict and implicates sRNAs as unidentified regulators of gene expression in phage-satellite interactions.
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影响因子：
64.5
作者：
Feng L;Rutherford ST;Papenfort K;Bagert JD;van Kessel JC;Tirrell DA;Wingreen NS;Bassler BL
通讯作者：
Bassler BL
影响因子：
11.3
作者：
通讯作者：
--
影响因子：
14.9
作者：
Barth, Zachary K.;Silvas, Tania V.;Seed, Kimberley D.
通讯作者：
Seed, Kimberley D.
影响因子：
11.3
作者：
Koskella B;Brockhurst MA
通讯作者：
Brockhurst MA
影响因子：
3.6
作者：
Davis, Brigid M.;Waldor, Matthew K.
通讯作者：
Waldor, Matthew K.