Tissue-Specific Transcriptomics Reveals an Important Role of the Unfolded Protein Response in Maintaining Fertility upon Heat Stress in Arabidopsis
Tissue-Specific Transcriptomics Reveals an Important Role of the Unfolded Protein Response in Maintaining Fertility upon Heat Stress in Arabidopsis
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组织特异性转录组学揭示未折叠蛋白反应在拟南芥热应激维持生育力中的重要作用
DOI：
10.1105/tpc.16.00916
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发表时间：
2017-04
期刊：
影响因子：
11.6
通讯作者：
Liu Jian-Xiang
中科院分区：
文献类型：
--
作者：
Zhang Shuang-Shuang;Yang Hongxing;Ding Lan;Song Ze-Ting;Ma Hong;Chang Fang;Liu Jian-Xiang
这项工作揭示了拟南芥在生殖阶段的组织特异性热响应和在热胁迫下维持生育力的重要UPR调节因子的下游基因。高温对植物生殖发育和随后的果实和种子的形成有很大的影响，但其潜在的分子机制还不清楚。我们使用转录组分析来研究热胁迫对拟南芥植物生殖发育的影响，并观察到营养组织与生殖组织的不同反应模式。暴露于热胁迫影响生殖发育程序，包括花药/胚珠发育和减数分裂的早期阶段。此外，参与未折叠蛋白反应（UPR）的基因在生殖组织特异性基因中富集，这些基因被热上调。此外，我们发现，UPR缺陷bzip 28 bzip 60双突变体是敏感的热胁迫，减少角果长度和生育力。热响应野生型与bzip 28 bzip 60植物的比较确定了521个基因的调控bZIP 28和bZIP 60在热胁迫下在生殖阶段，其中大部分是非典型的UPR基因。染色质免疫沉淀结合高通量测序分析揭示了133个可能的直接目标bZIP 28在拟南芥幼苗受到热胁迫，包括27个基因，也上调了热在生殖发育。我们的研究结果提供了重要的见解拟南芥生殖组织的热响应，并证明了保护作用的UPR维持生育力热胁迫。
This work reveals both the tissue-specific heat responsiveness of Arabidopsis at the reproductive stage and downstream genes of the UPR regulators important in maintaining fertility upon heat stress. High temperatures have a great impact on plant reproductive development and subsequent fruit and seed set, but the underlying molecular mechanisms are not well understood. We used transcriptome profiling to investigate the effect of heat stress on reproductive development of Arabidopsis thaliana plants and observed distinct response patterns in vegetative versus reproductive tissues. Exposure to heat stress affected reproductive developmental programs, including early phases of anther/ovule development and meiosis. Also, genes participating in the unfolded protein response (UPR) were enriched in the reproductive tissue-specific genes that were upregulated by heat. Moreover, we found that the UPR-deficient bzip28 bzip60 double mutant was sensitive to heat stresses and had reduced silique length and fertility. Comparison of heat-responsive wild type versus bzip28 bzip60 plants identified 521 genes that were regulated by bZIP28 and bZIP60 upon heat stress during reproductive stages, most of which were noncanonical UPR genes. Chromatin immunoprecipitation coupled with high-throughput sequencing analyses revealed 133 likely direct targets of bZIP28 in Arabidopsis seedlings subjected to heat stress, including 27 genes that were also upregulated by heat during reproductive development. Our results provide important insights into heat responsiveness in Arabidopsis reproductive tissues and demonstrate the protective roles of the UPR for maintaining fertility upon heat stress.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
27.5
作者：
Le Sun;Sun-Jie Lu;Shuang-Shuang Zhang-Shuang;Shun-Fan Zhou;Ling Sun;Jian-Xiang Liu
通讯作者：
Le Sun;Sun-Jie Lu;Shuang-Shuang Zhang-Shuang;Shun-Fan Zhou;Ling Sun;Jian-Xiang Liu
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
5.6
作者：
Bita CE;Gerats T
通讯作者：
Gerats T
影响因子：
7.4
作者：
Pina, C;Pinto, F;Becker, JD
通讯作者：
Becker, JD