Role for Astrocytes in mGluR-Dependent LTD in the Neocortex and Hippocampus.
Role for Astrocytes in mGluR-Dependent LTD in the Neocortex and Hippocampus.
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DOI：
10.3390/brainsci12121718
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发表时间：
2022-12-15
期刊：
影响因子：
3.3
通讯作者：
Pankratov, Yuriy
中科院分区：
文献类型：
--
作者：
Lalo, Ulyana;Pankratov, Yuriy
关键词：
星形胶质细胞是大脑可塑性的活性成分，能够通过多种机制释放小分子胶质递质，调节突触强度。虽然胶质-神经元通讯对长时程增强的重要性已被广泛报道，但对星形胶质细胞在长时程抑制（LTD）中的作用的研究才刚刚兴起。在这里，我们探讨了星形胶质细胞在突触可塑性的突出形式- mglur依赖性LTD中的作用。我们发现I组受体，特别是mGluR1亚型，在海马和新皮层星形胶质细胞中对Ca2+信号传导有重要贡献，可以在用于LTD诱导的突触刺激中激活。我们的数据表明，mGluR受体可以激活星形胶质细胞的snare依赖性ATP释放，进而可以直接激活海马和新皮层神经元的突触后P2X受体。后一种机制最近被证明是通过触发AMPA受体的内化而引起突触抑制的。利用神经胶质细胞分泌受损小鼠模型（dnSNARE小鼠），我们证明了mglur激活的星形胶质细胞释放ATP对CA3-CA1和2/3层突触中mglur依赖的LTD的调节是必不可少的。我们的数据还表明星形胶质细胞相关通路主要依赖于mGluR1受体，并与主要依赖于mGluR5的神经元机制协同作用。
Astroglia are an active element of brain plasticity, capable to release small molecule gliotransmitters by various mechanisms and regulate synaptic strength. While importance of glia-neuron communications for long-term potentiation has been rather widely reported, research into role for astrocytes in long-depression (LTD) is just gaining momentum. Here, we explored the role for astrocytes in the prominent form of synaptic plasticity—mGluR-dependent LTD. We found out the substantial contribution of the Group I receptors, especially mGluR1 subtype, into Ca2+-signaling in hippocampal and neocortical astrocytes, which can be activated during synaptic stimulation used for LTD induction. Our data demonstrate that mGluR receptors can activate SNARE-dependent release of ATP from astrocytes which in turn can directly activate postsynaptic P2X receptors in the hippocampal and neocortical neurons. The latter mechanism has recently been shown to cause the synaptic depression via triggering the internalisation of AMPA receptors. Using mouse model of impaired glial exocytosis (dnSNARE mice), we demonstrated that mGluR-activated release of ATP from astrocytes is essential for regulation of mGluR-dependent LTD in CA3-CA1 and layer 2/3 synapses. Our data also suggest that astrocyte-related pathway relies mainly on mGluR1 receptors and act synergistically with neuronal mechanisms dependent mainly on mGluR5.
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DOI：
10.1126/science.1190721
发表时间：
2010-07-30
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Gourine AV;Kasymov V;Marina N;Tang F;Figueiredo MF;Lane S;Teschemacher AG;Spyer KM;Deisseroth K;Kasparov S
通讯作者：
Kasparov S
影响因子：
9.8
作者：
Lalo U;Palygin O;Rasooli-Nejad S;Andrew J;Haydon PG;Pankratov Y
通讯作者：
Pankratov Y
哺乳动物海马缺血期间 ATP 和腺苷释放的时间和机械解离。
影响因子：
4.7
作者：
Frenguelli BG;Wigmore G;Llaudet E;Dale N
通讯作者：
Dale N
影响因子：
4.6
作者：
Lalo U;Palygin O;Verkhratsky A;Grant SG;Pankratov Y
通讯作者：
Pankratov Y
影响因子：
5.3
作者：
Pankratov Y;Lalo U
通讯作者：
Lalo U