LcMYB1 is a key determinant of differential anthocyanin accumulation among genotypes, tissues, developmental phases and ABA and light stimuli in Litchi chinensis.
LcMYB1 is a key determinant of differential anthocyanin accumulation among genotypes, tissues, developmental phases and ABA and light stimuli in Litchi chinensis.
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DOI：
10.1371/journal.pone.0086293
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Hu GB
中科院分区：
文献类型：
--
作者：
Lai B;Li XJ;Hu B;Qin YH;Huang XM;Wang HC;Hu GB
荔枝果实的红色取决于花青素的积累。不同品种、不同发育阶段、不同环境刺激条件下荔枝果实花色苷含量差异较大。以往对多种植物的研究表明，花青素的生物合成在转录水平上受到控制。在这里，我们描述了荔枝R2R3-MYB转录因子基因LcMYB1，它显示了与其他已知花青素调节因子相似的序列。研究了不同花色苷含量的样品中LcMYB1和花青素生物合成基因的转录水平。LcMYB1的表达与组织花色苷含量密切相关。LcMYB1转录本仅在花青素积累组织中检测到，且与果皮花青素积累呈正相关。ABA和日照促进了果皮LcMYB1的表达，而CPPU和套袋则抑制了LcMYB1的表达和花青素的积累。在LcMYB1的启动子区域发现了与光反应和脱落酸反应相关的顺式元件。在检测的6个结构基因中，只有LcUFGT与LcMYB1高度相关。这些结果表明，LcMYB1控制荔枝花青素的生物合成，LcUFGT可能是LcMYB1靶向调控的结构基因。此外，LcMYB1的过表达诱导了烟草各组织中花青素的积累，证实了LcMYB1在花青素生物合成中的调节功能。LcMYB1过表达对NtAn1b的上调似乎对花青素在叶片和花梗中的积累是必不可少的。然而，在转基因烟草的生殖组织中，花青素积累的增加与烟草内源MYB和bHLH转录因子无关，但与特定结构基因的上调有关。
The red coloration of litchi fruit depends on the accumulation of anthocyanins. The anthocyanins level in litchi fruit varies widely among cultivars, developmental stages and environmental stimuli. Previous studies on various plant species demonstrate that anthocyanin biosynthesis is controlled at the transcriptional level. Here, we describe a litchi R2R3-MYB transcription factor gene, LcMYB1, which demonstrates a similar sequence as other known anthocyanin regulators. The transcription levels of the LcMYB1 and anthocyanin biosynthetic genes were investigated in samples with different anthocyanin levels. The expression of LcMYB1 was strongly associated with tissue anthocyanin content. LcMYB1 transcripts were only detected in anthocyanin-accumulating tissues and were positively correlated with anthocyanin accumulation in the pericarps of 12 genotypes. ABA and sunlight exposure promoted, whereas CPPU and bagging inhibited the expression of LcMYB1 and anthocyanin accumulation in the pericarp. Cis-elements associated with light responsiveness and abscisic acid responsiveness were identified in the promoter region of LcMYB1. Among the 6 structural genes tested, only LcUFGT was highly correlated with LcMYB1. These results suggest that LcMYB1 controls anthocyanin biosynthesis in litchi and LcUFGT might be the structural gene that is targeted and regulated by LcMYB1. Furthermore, the overexpression of LcMYB1 induced anthocyanin accumulation in all tissues in tobacco, confirming the function of LcMYB1 in the regulation of anthocyanin biosynthesis. The upregulation of NtAn1b in response to LcMYB1 overexpression seems to be essential for anthocyanin accumulation in the leaf and pedicel. In the reproductive tissues of transgenic tobacco, however, increased anthocyanin accumulation is independent of tobacco's endogenous MYB and bHLH transcriptional factors, but associated with the upregulation of specific structural genes.
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DOI：
10.1111/j.1365-313x.2006.02964.x
发表时间：
2007-02
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Espley RV;Hellens RP;Putterill J;Stevenson DE;Kutty-Amma S;Allan AC
通讯作者：
Allan AC
影响因子：
7.4
作者：
Deluc, L;Barrieu, F;Hamdi, S
通讯作者：
Hamdi, S
影响因子：
5.1
作者：
Boss, PK;Davies, C;Robinson, SP
通讯作者：
Robinson, SP
影响因子：
7.4
作者：
Chagne, David;Kui Lin-Wang;Allan, Andrew C.
通讯作者：
Allan, Andrew C.
影响因子：
3.9
作者：
FULEKI, T;FRANCIS, FJ
通讯作者：
FRANCIS, FJ