Neural pathfinding on uni- and multidirectional photopolymerized micropatterns.
Neural pathfinding on uni- and multidirectional photopolymerized micropatterns.
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DOI：
10.1021/am501622a
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发表时间：
2014-07-23
影响因子：
9.5
通讯作者：
Guymon, C. Allan
中科院分区：
文献类型：
--
作者：
Tuft, Bradley W.;Xu, Linjing;White, Scott P.;Seline, Alison E.;Erwood, Andrew M.;Hansen, Marian R.;Guymon, C. Allan
克服神经假体的信号分辨率障碍，例如市售的人工耳蜗（CI）或正在开发的视网膜植入物，可能需要对再生神经元件进行空间控制。为了合理设计引导神经生长的材料，首先需要确定内耳螺旋神经节神经元（SGN）等假体相关细胞的从头神经突生长的寻路行为。因此，在这项工作中，重复的 90° 转动被制造为多向微图案，以确定 SGN 神经突转动能力和寻路能力。使用单向微图案和无图案基材作为比较。还在每种表面类型上检查螺旋神经节雪旺细胞排列 (SGSC)。使用光掩模光聚合固有的空间反应控制来制造微图案，光掩模具有用于单向图案的平行线间距光栅或用于多向图案的重复90°角步骤。通过以给定时间增量关闭光源来调节 UV 曝光时间来控制特征深度。通过白光干涉测量法和扫描电子显微镜 (SEM) 来表征基板形貌。两种图案类型均具有宽度为 25 μm、深度为 7.4 ± 0.7 μm 的特征。 SGN 神经突在未图案化的光聚合物控制上随机定向，对齐并一致地跟踪单向图案，并且很大程度上受到多向转动线索的影响，但并不一致地跟踪。与单向图案相比，多向基底上的神经突长度短 20%，而神经突分支和微观特征交叉事件明显更高。对于两种图案类型，大部分神经突长度位于凹陷的表面特征中。开发理解神经寻路并引导神经突从头生长到特定刺激元素的方法将有助于设计创新的生物材料，从而改善与神经系统接口的设备的功能结果。
Overcoming signal resolution barriers of neural prostheses, such as the commercially available cochlear impant (CI) or the developing retinal implant, will likely require spatial control of regenerative neural elements. To rationally design materials that direct nerve growth, it is first necessary to determine pathfinding behavior of de novo neurite growth from prosthesis-relevant cells such as spiral ganglion neurons (SGNs) in the inner ear. Accordingly, in this work, repeating 90° turns were fabricated as multidirectional micropatterns to determine SGN neurite turning capability and pathfinding. Unidirectional micropatterns and unpatterned substrates are used as comparisons. Spiral ganglion Schwann cell alignment (SGSC) is also examined on each surface type. Micropatterns are fabricated using the spatial reaction control inherent to photopolymerization with photomasks that have either parallel line spacing gratings for unidirectional patterns or repeating 90° angle steps for multidirectional patterns. Feature depth is controlled by modulating UV exposure time by shuttering the light source at given time increments. Substrate topography is characterized by white light interferometry and scanning electron microscopy (SEM). Both pattern types exhibit features that are 25 μm in width and 7.4 ± 0.7 μm in depth. SGN neurites orient randomly on unpatterned photopolymer controls, align and consistently track unidirectional patterns, and are substantially influenced by, but do not consistently track, multidirectional turning cues. Neurite lengths are 20% shorter on multidirectional substrates compared to unidirectional patterns while neurite branching and microfeature crossing events are significantly higher. For both pattern types, the majority of the neurite length is located in depressed surface features. Developing methods to understand neural pathfinding and to guide de novo neurite growth to specific stimulatory elements will enable design of innovative biomaterials that improve functional outcomes of devices that interface with the nervous system.
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影响因子：
2.8
作者：
Clarke, Joseph C.;Tuft, Bradley W.;Clinger, John D.;Levine, Rachel;Figueroa, Lucas Sievens;Guymon, C. Allan;Hansen, Marlan R.
通讯作者：
Hansen, Marlan R.
影响因子：
14
作者：
Bryant, Stephanie J.;Cuy, Janet L.;Ratner, Buddy D.
通讯作者：
Ratner, Buddy D.
影响因子：
14
作者：
Branch, DW;Wheeler, BC;Leckband, DE
通讯作者：
Leckband, DE
影响因子：
14
作者：
Johansson, F;Carlberg, P;Kanje, M
通讯作者：
Kanje, M
影响因子：
14
作者：
Cui, XY;Wiler, J;Martin, DC
通讯作者：
Martin, DC