Micropatterned methacrylate polymers direct spiral ganglion neurite and Schwann cell growth.
Micropatterned methacrylate polymers direct spiral ganglion neurite and Schwann cell growth.
复制标题
DOI：
10.1016/j.heares.2011.05.004
复制
发表时间：
2011-08
期刊：
影响因子：
2.8
通讯作者：
Hansen, Marlan R.
中科院分区：
文献类型：
--
作者：
Clarke, Joseph C.;Tuft, Bradley W.;Clinger, John D.;Levine, Rachel;Figueroa, Lucas Sievens;Guymon, C. Allan;Hansen, Marlan R.
人工耳蜗植入术在功能方面的显著进步可能需要组织工程方法来改善神经假体界面。一种策略是以高度组织化的方式引导螺旋神经节神经元（SGN）轴突生长以接近或接触刺激电极。在这里，我们评估了甲基丙烯酸酯（MA）聚合物系统中光聚合诱导的微图案直接培养新生大鼠SGN神经突生长和SG雪旺细胞（SGSC）排列的能力。SGN存活和神经突长度在各种聚合物组合物之间是相当的。值得注意的是，在层粘连蛋白和非层粘连蛋白涂覆的MA聚合物基底之间，SGN存活或神经突长度没有显著差异，表明与SG组织具有高生物相容性。利用光聚合的微图案化产生具有50 μm的脊周期性和0.6-1.0 μm的通道深度的微通道。SGN神经突起在微通道的凹槽内生长。这些拓扑结构强烈诱导解离的SGN神经突和SGSC的排列以平行于图案。相比之下，成纤维细胞未能与微图案对齐，表明细胞对地形线索的特异性反应。SGN神经突从外植体延伸到平行的模式，因为他们遇到的微通道。转向的程度与神经突最初遇到图案的角度显著相关。这些结果表明，SGN神经突响应于微地形特征，并且这些特征可以用于以高度组织化的方式指导神经突生长。
Significant advances in the functional outcomes achieved with cochlear implantation will likely require tissue-engineering approaches to improve the neural prosthesis interface. One strategy is to direct spiral ganglion neuron (SGN) axon growth in a highly organized fashion to approximate or contact stimulating electrodes. Here we assessed the ability of micropatterns induced by photopolymerization in methacrylate (MA) polymer systems to direct cultured neonatal rat SGN neurite growth and alignment of SG Schwann cells (SGSCs). SGN survival and neurite length were comparable among various polymer compositions. Remarkably, there was no significant difference in SGN survival or neurite length between laminin and non-laminin coated MA polymer substrates, suggesting high biocompatibility with SG tissue. Micropatterning with photopolymerization generated microchannels with a ridge periodicity of 50 µm and channel depths of 0.6–1.0 µm. SGN neurites grew within the grooves of the microchannels. These topographies strongly induced alignment of dissociated SGN neurites and SGSCs to parallel the pattern. By contrast, fibroblasts failed to align with the micropattern suggesting cell specific responses to topographical cues. SGN neurites extending from explants turned to parallel the pattern as they encountered the microchannels. The extent of turning was significantly correlated with angle at which the neurite initially encountered the pattern. These results indicate that SGN neurites respond to microtopographical features and that these features can be used to direct neurite growth in a highly organized fashion.
登录
查看更多内容
影响因子：
14
作者：
Bryant, Stephanie J.;Cuy, Janet L.;Ratner, Buddy D.
通讯作者：
Ratner, Buddy D.
影响因子：
2.5
作者：
Brors, D;Bodmer, D;Ryan, AF
通讯作者：
Ryan, AF
影响因子：
14
作者：
Branch, DW;Wheeler, BC;Leckband, DE
通讯作者：
Leckband, DE
影响因子：
14
作者：
Johansson, F;Carlberg, P;Kanje, M
通讯作者：
Kanje, M
影响因子：
9
作者：
Fozdar DY;Lee JY;Schmidt CE;Chen S
通讯作者：
Chen S