Susceptibility of the wild-derived inbred CAST/Ei mouse to infection by orthopoxviruses analyzed by live bioluminescence imaging.
Susceptibility of the wild-derived inbred CAST/Ei mouse to infection by orthopoxviruses analyzed by live bioluminescence imaging.
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DOI：
10.1016/j.virol.2013.11.017
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发表时间：
2014-01-20
期刊：
影响因子：
3.7
通讯作者：
Earl, Patricia L.
中科院分区：
文献类型：
--
作者：
Americo, Jeffrey L.;Sood, Cindy L.;Cotter, Catherine A.;Vogel, Jodi L.;Kristie, Thomas M.;Moss, Bernard;Earl, Patricia L.
关键词：
经典的近交系小鼠被广泛用于病毒研究。然而，我们最近发现，一些野生来源的近交系小鼠品系比经典品系对猴痘病毒更敏感。这里描述的实验表明，牛痘病毒（VACV）和牛痘病毒（CPXV）的50%致死剂量在野生型近交系CAST/Ei小鼠中比经典近交系BALB/c小鼠低两个对数，而小鼠品系对单纯疱疹病毒的易感性几乎没有差异。活体生物发光成像用于追踪致病性和减毒VACV毒株和CPXV病毒从CAST/Ei小鼠的鼻道到胸部和腹部器官的传播。发光首先在头部增加，然后以剂量依赖性方式同时在胸部和腹部增加。尽管峰值光子通量相似，但VACV的扩散动力学比CPXV更快。这些数据表明CAST/Ei小鼠用于正痘病毒研究的优势。
Classical inbred mice are extensively used for virus research. However, we recently found that some wild-derived inbred mouse strains are more susceptible than classical strains to monkeypox virus. Experiments described here indicated that the 50% lethal dose of vaccinia virus (VACV) and cowpox virus (CPXV) were two logs lower in wild-derived inbred CAST/Ei mice than classical inbred BALB/c mice, whereas there was little difference in the susceptibility of the mouse strains to herpes simplex virus. Live bioluminescence imaging was used to follow spread of pathogenic and attenuated VACV strains and CPXV virus from nasal passages to organs in the chest and abdomen of CAST/Ei mice. Luminescence increased first in the head and then simultaneously in the chest and abdomen in a dose-dependent manner. The spreading kinetics was more rapid with VACV than CPXV although the peak photon flux was similar. These data suggest advantages of CAST/Ei mice for orthopoxvirus studies.
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