Sbg1 Is a Novel Regulator for the Localization of the β-Glucan Synthase Bgs1 in Fission Yeast.
Sbg1 Is a Novel Regulator for the Localization of the β-Glucan Synthase Bgs1 in Fission Yeast.
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DOI：
10.1371/journal.pone.0167043
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Wu JQ
中科院分区：
文献类型：
--
作者：
Davidson R;Pontasch JA;Wu JQ
葡聚糖脱氢酶合成葡聚糖，复杂的多糖，是真菌细胞壁和分裂隔膜的主要成分。研究葡聚糖糖苷酶的调节是重要的，因为它们是真菌细胞生存所必需的，因此是抗真菌药物的流行靶点。线性1，3-β-葡聚糖是初生隔膜的主要成分，由裂殖酵母胞质分裂中保守的β-葡聚糖合成酶Bgs 1合成。已知Rho 1 GTdR调节Bgs 1催化活性，并且F-BAR蛋白Cdc 15在Bgs 1递送至质膜中起作用。在这里，我们表征了一种新的蛋白Sbg 1，它存在于与Bgs 1的复合物中，并调节其蛋白水平和定位。在胞质分裂过程中，sbg 1对收缩环的收缩和隔膜的形成至关重要。Sbg 1和Bgs 1在物理上相互作用，并相互依赖，定位于质膜。在sbg 1突变体中，bgs 1不太稳定和/或错误地靶向液泡。此外，Sbg 1在Bgs 1运输和定位中比Cdc 15起更早和更重要的作用。总之，我们的数据揭示了新蛋白Sbg 1对必需β-葡聚糖合酶Bgs 1的新调控模式。
Glucan synthases synthesize glucans, complex polysaccharides that are the major components in fungal cell walls and division septa. Studying regulation of glucan synthases is important as they are essential for fungal cell survival and thus popular targets for anti-fungal drugs. Linear 1,3-β-glucan is the main component of primary septum and is synthesized by the conserved β-glucan synthase Bgs1 in fission yeast cytokinesis. It is known that Rho1 GTPase regulates Bgs1 catalytic activity and the F-BAR protein Cdc15 plays a role in Bgs1 delivery to the plasma membrane. Here we characterize a novel protein Sbg1 that is present in a complex with Bgs1 and regulates its protein levels and localization. Sbg1 is essential for contractile-ring constriction and septum formation during cytokinesis. Sbg1 and Bgs1 physically interact and are interdependent for localization to the plasma membrane. Bgs1 is less stable and/or mis-targeted to vacuoles in sbg1 mutants. Moreover, Sbg1 plays an earlier and more important role in Bgs1 trafficking and localization than Cdc15. Together, our data reveal a new mode of regulation for the essential β-glucan synthase Bgs1 by the novel protein Sbg1.
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影响因子：
7.8
作者：
Coffman, Valerie C.;Sees, Jennifer A.;Wu, Jian-Qiu
通讯作者：
Wu, Jian-Qiu
影响因子：
8.8
作者：
Arasada, Rajesh;Pollard, Thomas D.
通讯作者：
Pollard, Thomas D.
DOI：
10.1083/jcb.201202015
发表时间：
2012-08-20
期刊：
The Journal of cell biology
影响因子：
--
作者：
Cortés JC;Sato M;Muñoz J;Moreno MB;Clemente-Ramos JA;Ramos M;Okada H;Osumi M;Durán A;Ribas JC
通讯作者：
Ribas JC
影响因子：
--
作者：
Estravis, Miguel;Rincon, Sergio;Perez, Pilar
通讯作者：
Perez, Pilar
影响因子：
56.9
作者：
Drgonova, J;Drgon, T;Cabib, E
通讯作者：
Cabib, E