A widespread and unusual RNA trans-splicing type in dinoflagellate mitochondria.
A widespread and unusual RNA trans-splicing type in dinoflagellate mitochondria.
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DOI：
10.1371/journal.pone.0056777
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Waller RF
中科院分区：
文献类型：
--
作者：
Jackson CJ;Waller RF
细胞色素氧化酶亚基3（cytochrome oxidase subunit 3，Cox3）是线粒体呼吸链复合体IV的核心膜蛋白，由7个跨膜螺旋组成。在这里，我们表明，在不同的后分支甲藻中，cox 3在线粒体基因组中螺旋6和7之间一致地分裂为两个外显子。基因外显子被转录为两个离散的寡腺苷酸化前体RNA，并且这些RNA随后被反式剪接以形成完整的编码mRNA。这种反式剪接是非常不寻常的，因为一些寡聚腺苷酸化的尾部在剪接位点被掺入，使得一短串腺苷连接两个编码外显子。这一特征在不同的腰鞭毛虫中始终存在，但是根据两个外显子之间的编码间隙的大小，所添加的腺苷的数量不同。因此，我们观察到0（Amphidinium carterae）和10（Symbiodinium sp.）在不同的分类群中添加了腺苷，但最终编码序列长度与保持的阅读框架相同。北方分析表明，前体cox 3转录物与成熟cox 3 mRNA的丰度大致相等，表明成熟过程缓慢或受调控。这些数据表明，在甲藻线粒体的剪接机制是宽容的前体编码序列的长度的变化，并暗示使用的剪接模板，或指导分子，在剪接过程中控制成熟的mRNA长度。
Cytochrome oxidase subunit 3 (Cox3) is a mitochondrion-encoded core membrane protein of complex IV of the mitochondrial respiratory chain, and consists of seven trans-membrane helices. Here we show that in diverse later-branching dinoflagellates, cox3 is consistently split into two exons in the mitochondrial genome between helices six and seven. Gene exons are transcribed as two discrete oligoadenylated precursor RNAs, and these are subsequently trans-spliced to form a complete coding mRNA. This trans-splicing is highly unusual in that some of the oligoadenylated tail is incorporated at the splice site, such that a short string of adenosines links the two coding exons. This feature is consistently represented in diverse dinoflagellates, however the number of adenosines added varies according to the size of the coding gap between the two exons. Thus we observed between zero (Amphidinium carterae) and 10 (Symbiodinium sp.) adenosines added in different taxa, but the final coding sequence length is identical with the reading frame maintained. Northern analyses show that precursor cox3 transcripts are approximately equally abundant as mature cox3 mRNAs, suggesting a slow or regulated maturation process. These data indicate that the splicing mechanism in dinoflagellate mitochondria is tolerant of variations in the length of the precursor coding sequence, and implicates the use of a splicing template, or guide molecule, during splicing that controls mature mRNA length.
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影响因子：
14.9
作者：
Gillespie, DE;Salazar, NA;Feagin, JE
通讯作者：
Feagin, JE
影响因子：
3.3
作者：
Jackson CJ;Gornik SG;Waller RF
通讯作者：
Waller RF
DOI：
10.1073/pnas.151268698
发表时间：
2001-08-14
影响因子：
11.1
作者：
Dorn, R;Reuter, G;Loewendorf, A
通讯作者：
Loewendorf, A
通过剪接体介导的反式剪接切除肠贾第鞭毛虫基因组中的分裂内含子
影响因子：
9.2
作者：
Kamikawa, Ryoma;Inagaki, Yuji;Hashimoto, Tetsuo
通讯作者：
Hashimoto, Tetsuo
影响因子：
11.4
作者：
Burger, Gertraud;Yan, Yifei;Lang, B. Franz
通讯作者：
Lang, B. Franz