Combined biophysical and soluble factor modulation induces cardiomyocyte differentiation from human muscle derived stem cells.
Combined biophysical and soluble factor modulation induces cardiomyocyte differentiation from human muscle derived stem cells.
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DOI：
10.1038/srep06614
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发表时间：
2014-10-14
影响因子：
4.6
通讯作者：
Tobita K
中科院分区：
文献类型：
--
作者：
Tchao J;Han L;Lin B;Yang L;Tobita K
Cellular cardiomyoplasty has emerged as a novel therapy to restore contractile function of injured failing myocardium. Human multipotent muscle derived stem cells (MDSC) can be a potential abundant, autologous cell source for cardiac repair. However, robust conditions for cardiomyocyte (CM) differentiation are not well established for this cell type. We have developed a new method for CM differentiation from human MDSC that combines 3-dimensional artificial muscle tissue (AMT) culture with temporally controlled biophysical cell aggregation and delivery of 4 soluble factors (microRNA-206 inhibitor, IWR-1, Lithium Chloride, and BMP-4) (4F-AG-AMT). The 4F-AG-AMT displayed cardiac-like response to β-adrenergic stimulation and contractile properties. 4F-AG-AMT expressed major cardiac (NKX2-5, GATA4, TBX5, MEF2C) transcription factors and structural proteins. They also express cardiac gap-junction protein, connexin-43, similar to CMs and synchronized spontaneous calcium transients. These results highlight the importance of temporal control of biophysical and soluble factors for CM differentiation from MDSCs.
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影响因子：
3.7
作者：
Clause KC;Tchao J;Powell MC;Liu LJ;Huard J;Keller BB;Tobita K
通讯作者：
Tobita K
影响因子：
5
作者：
Grajales L;García J;Geenen DL
通讯作者：
Geenen DL
DOI：
10.1186/1478-811x-7-4
发表时间：
2009-03-12
期刊：
Cell communication and signaling : CCS
影响因子：
--
作者：
Dbouk HA;Mroue RM;El-Sabban ME;Talhouk RS
通讯作者：
Talhouk RS
影响因子：
6.6
作者：
Guo, Xiufang;Greene, Keshel;Hickman, James J.
通讯作者：
Hickman, James J.
影响因子：
--
作者：
Friedrichs M;Wirsdöerfer F;Flohé SB;Schneider S;Wuelling M;Vortkamp A
通讯作者：
Vortkamp A