Characterisation of the Paternal Influence on Intergenerational Offspring Cardiac and Brain Lipid Homeostasis in Mice.
Characterisation of the Paternal Influence on Intergenerational Offspring Cardiac and Brain Lipid Homeostasis in Mice.
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DOI：
10.3390/ijms24031814
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发表时间：
2023-01-17
影响因子：
5.6
通讯作者：
Watkins, Adam J.
中科院分区：
文献类型：
--
作者：
Furse, Samuel;Morgan, Hannah L.;Koulman, Albert;Watkins, Adam J.
越来越多的证据表明，怀孕时父亲的不良饮食会增加子女成年后患上一系列非传染性代谢性疾病的风险，如肥胖症、糖尿病和心血管疾病。我们假设，父亲的低蛋白质-高碳水化合物饮食通过精子和精浆介导的机制扰乱了后代组织的脂肪丰度。为了验证我们的假设，我们给雄性C57BL/6小鼠喂以正常蛋白质饮食(NPD；18%蛋白质)或等卡路里低蛋白质饮食(LPD；9%蛋白质)至少8周。我们通过人工授精结合输精管结扎的雄性交配产生了后代。使用这种方法，我们从NPD或LPD精子中获得了后代，但在NPD或LPD精浆存在的情况下。使用高分辨率质谱仪，我们发现来自LPD精子或精液的后代从三周大开始就表现出心脏和大脑脂质丰度的扰动，通常与组织甘油三酯丰度的变化有关。我们还观察了对照组和试验组后代心脏和大脑中不同性别的血脂模式。这些观察表明，怀孕时父亲不良的饮食会以特定于年龄、性别和世代的方式影响后代的心脏和大脑脂肪谱。
There is growing evidence that poor paternal diet at the time of conception increase the risk of offspring developing a range of non-communicable metabolic diseases, such as obesity, diabetes and cardiovascular disease, in adulthood. We hypothesise that a paternal low protein–high carbohydrate diet perturbs offspring tissue lipid abundance through both sperm and seminal plasma-mediated mechanisms. To test our hypothesis, we fed male C57BL/6 mice either a control normal protein diet (NPD; 18% protein) or an isocaloric low protein diet (LPD; 9% protein) for a minimum of 8 weeks. We generated offspring through artificial insemination, in combination with vasectomised male mating. Using this approach, we derived offspring from either NPD or LPD sperm but in the presence of NPD or LPD seminal plasma. Using high resolution mass-spectrometry, we found that offspring derived from either LPD sperm or seminal fluid displayed perturbed cardiac and brain lipid abundance from just three weeks of age, typically associated with the altered abundance of tissue triglycerides. We also observed the differential sex-specific patterns of lipids between the control and experimental offspring’s hearts and brains. These observations indicate that poor paternal diet at the time of conception affects offspring cardiac and brain lipid profiles in an age-, sex- and generation-specific manner.
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影响因子：
64.5
作者：
Carone BR;Fauquier L;Habib N;Shea JM;Hart CE;Li R;Bock C;Li C;Gu H;Zamore PD;Meissner A;Weng Z;Hofmann HA;Friedman N;Rando OJ
通讯作者：
Rando OJ
DOI：
10.1007/s12154-012-0090-1
发表时间：
2013-04
期刊：
Journal of chemical biology
影响因子：
--
作者：
Furse, Samuel;Liddell, Susan;Ortori, Catharine A;Williams, Huw;Neylon, D Cameron;Scott, David J;Barrett, David A;Gray, David A
通讯作者：
Gray, David A
影响因子：
5.9
作者：
Furse S;Watkins AJ;Hojat N;Smith J;Williams HEL;Chiarugi D;Koulman A
通讯作者：
Koulman A
十年前瞻性队列研究中脂质谱与心血管疾病和死亡的关联
影响因子：
3.6
作者：
Dong J;Yang S;Zhuang Q;Sun J;Wei P;Zhao X;Chen Y;Chen X;Li M;Wei L;Chen C;Fan Y;Shen C
通讯作者：
Shen C
DOI：
10.1017/s2040174416000477
发表时间：
2016-10-01
影响因子：
1.7
作者：
Dickinson, H.;Moss, T. J.;Morrison, J. L.
通讯作者：
Morrison, J. L.