Nitrogen-Regulated Interactions Between Microcystis aeruginosa and Spiramycin Contaminant
Nitrogen-Regulated Interactions Between Microcystis aeruginosa and Spiramycin Contaminant
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铜绿微囊藻和螺旋霉素污染物之间氮调节的相互作用
DOI：
10.1007/s11270-015-2412-8
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发表时间：
2015-04
期刊：
影响因子：
--
通讯作者：
Gao, Baoyu
中科院分区：
文献类型：
--
作者：
Liu, Ying;Chen, Shi;Zhang, Jian;Gao, Baoyu
在7天的暴露试验中，氮显著调节了螺旋霉素对铜绿微囊藻的生长和抗氧化反应以及对螺旋霉素的生物降解的影响(p <； 0.05)。在L−1施氮量为0.5 mg的条件下，10 0~4 0 0 mg的L−1可刺激超氧化物歧化酶和过氧化氢酶的活性，保护藻细胞免受氧化损伤，从而减轻螺旋霉素的毒性，降低铜绿假单胞菌的丙二醛含量。在较高的氮素水平(5~50 mg)下，L−1的400 ng螺旋霉素对−1的过氧化氢酶活性有明显的抑制作用(p <； 0.05)，并通过过氧化氢的积累增加丙二醛的含量。激活的谷胱甘肽含量和谷胱甘肽S转移酶活性与铜绿假单胞菌对螺旋霉素的降解有关。螺旋霉素的7d降解率在8.9%~29.6%之间，随着氮素浓度的增加和螺旋霉素浓度的降低而增加。根据发光细菌试验，由于对藻类生长的调节，L 1 100ng/ −1，氮浓度0.5g/L−1，对铜绿假单胞菌的毒性显著增强(p <； 0.05)，而L−1,400ng，氮水平5-50 mg/L−1，显著降低(p <； 0.05)。在控制铜绿假单胞菌水华的过程中，尤其是在缺氮条件下，应考虑低浓度的螺旋霉素共存污染物。
Nitrogen significantly regulated (p < 0.05) the effects of spiramycin on the growth and antioxidant responses of Microcystis aeruginosa as well as the biodegradation of spiramycin by M. aeruginosa during a 7-day exposure test. At a nitrogen level of 0.5 mg L−1, the activities of superoxide dismutase and catalase were stimulated by 100–400 ng L−1 of spiramycin to protect algal cells from oxidative damage, resulting in alleviated toxicity of spiramycin and low malondialdehyde content in M. aeruginosa. The catalase activity was inhibited by 400 ng L−1 of spiramycin at higher nitrogen levels of 5–50 mg L−1, leading to significant growth inhibition (p < 0.05) and higher malondialdehyde content through accumulation of hydrogen peroxide. Stimulated glutathione content and glutathione S-transferase activity were coupled to the biodegradation of spiramycin in M. aeruginosa. The 7-day biodegradation percentage of spiramycin varied from 8.9 to 29.6 %, which was enhanced by increased nitrogen concentration and decreased spiramycin concentration. Due to the regulation of algal growth, the toxicity of M. aeruginosa were significantly enhanced (p < 0.05) by 100 ng L−1 of spiramycin at a nitrogen concentration of 0.5 mg L−1 while significantly reduced (p < 0.05) by 400 ng L−1 of spiramycin at nitrogen levels of 5–50 mg L−1, according to the luminescent bacteria test. Low concentration of coexisting spiramycin contaminant should be considered during the control of M. aeruginosa bloom, especially under nitrogen deficient condition.
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蓝藻铜绿微囊藻对多酚应激的抗氧化和代谢反应
DOI：
10.1080/03601234.2013.727722
发表时间：
2013-01
期刊：
Journal of Environmental Science and Health - Part B: Pesticides, Food Contaminants, and Agricultural Wastes
影响因子：
--
作者：
Ni, Li X.;Acharya, Kumud;Hao, Xiang Y.;Li, Shi Y.
通讯作者：
Li, Shi Y.
影响因子：
1.5
作者：
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通讯作者：
P. Bhargava;N. Atri;A. Srivastava;L. Rai
影响因子：
8.8
作者：
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通讯作者：
Zhiquan Liu;F. Cui;Hua Ma;Zhenqiang Fan;Zhiwei Zhao;Z. Hou;Dongmei Liu
影响因子：
11.4
作者：
CAIRNS, J;MOUNT, DI
通讯作者：
MOUNT, DI
影响因子：
6.8
作者：
T. Stoichev;M. S. Baptista;M. C. Basto;V. Vasconcelos;M. Vasconcelos
通讯作者：
T. Stoichev;M. S. Baptista;M. C. Basto;V. Vasconcelos;M. Vasconcelos