Analysis of minimal promoter sequences for plus-strand synthesis by the Cucumber necrosis virus RNA-dependent RNA polymerase.
Analysis of minimal promoter sequences for plus-strand synthesis by the Cucumber necrosis virus RNA-dependent RNA polymerase.
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黄瓜坏死病毒 RNA 依赖性 RNA 聚合酶正链合成的最小启动子序列分析。
作者：
T. Panavas;J. Pogány;P. D. Nagy
Tombusviruses是植物的小的、正义的、单链RNA病毒。利用部分纯化的黄瓜坏死病毒（CNV）RNA依赖性RNA聚合酶（RdRp），从正链或负链模板出发，重新启动互补RNA的合成。体外RdRp分析表明，核心正链起始启动子仅包含3 '端11个核苷酸。假设的启动子样序列，其已被Wu和白色（1998，J. Virol. 72，9897-9905）被CNV RdRp识别的效率低于核心正链起始启动子。CNV RdRp可以有效识别与远缘芜菁皱缩病毒相关的卫星RNA的核心正链起始启动子，而人工富含Au或GC的3 '端序列在体外测定中是较差的模板。最小的正链和负链起始启动子的“强度”的比较表明，后者在促进互补RNA合成方面的效率几乎是前者的两倍。然而，模板竞争实验表明，最小正链起始启动子使RNA模板比最小负链起始启动子更具竞争力。综上所述，这些结果表明，通过番茄丛矮病毒RdRp的启动子识别仅需要存在于模板的3'端的短序列。
Tombusviruses are small, plus-sense, single-stranded RNA viruses of plants. A partially purified RNA-dependent RNA polymerase (RdRp) preparation of Cucumber necrosis virus (CNV), which is capable of de novo initiation of complementary RNA synthesis from either plus-strand or minus-strand templates, was used to dissect minimal promoter sequences for tombusviruses and their defective interfering (DI) RNAs. In vitro RdRp assay revealed that the core plus-strand initiation promoter included only the 3'-terminal 11 nucleotides. A hypothetical promoter-like sequence, which has been termed consensus sequence by Wu and White (1998, J. Virol. 72, 9897-9905), is recognized less efficiently by the CNV RdRp than the core plus-strand initiation promoter. The CNV RdRp can efficiently recognize the core plus-strand initiation promoter for a satellite RNA associated with the distantly related Turnip crinkle virus, while artificial AU- or GC-rich 3'-terminal sequences make poor templates in the in vitro assays. Comparison of the "strength" of minimal plus-strand and minus-strand initiation promoters reveals that the latter is almost twice as efficient in promoting complementary RNA synthesis. Template competition experiments, however, suggest that the minimal plus-strand initiation promoter makes an RNA template more competitive than the minimal minus-strand initiation promoter. Taken together, these results demonstrate that promoter recognition by the tombusvirus RdRp requires only short sequences present at the 3' end of templates.
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没有独特启动子元件的 CCA 起始盒支持三种病毒 RNA 依赖性 RNA 聚合酶的体外转录。
DOI：
10.1017/s1355838200992410
发表时间：
2000
期刊：
RNA (New York, N.Y.)
影响因子：
--
作者：
Yoshinari,S;Nagy,PD;Simon,AE;Dreher,TW
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Dreher,TW
芜菁黄花叶病毒RNA依赖性RNA聚合酶：体外负链合成的起始。
DOI：
10.1006/viro.1997.8621
发表时间：
1997
期刊：
Virology.
影响因子：
--
作者：
Singh,RN;Dreher,TW
通讯作者：
Dreher,TW
由非特异性二级结构和-CCR-盒组合产生的RNA中的特异性位点选择：萝卜黄花叶病毒RNA依赖性RNA聚合酶启动负链合成。
DOI：
10.1017/s1355838298980694
发表时间：
1998
期刊：
RNA (New York, N.Y.)
影响因子：
--
作者：
Singh,RN;Dreher,TW
通讯作者：
Dreher,TW
DOI：
10.1073/pnas.90.4.1498
发表时间：
1993-02-15
影响因子：
11.1
作者：
QUADT, R;KAO, CC;AHLQUIST, P
通讯作者：
AHLQUIST, P