Functional markers developed from TaGS3, a negative regulator of grain weight and size, for marker-assisted selection in wheat
Functional markers developed from TaGS3, a negative regulator of grain weight and size, for marker-assisted selection in wheat
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从 TaGS3（籽粒重量和大小的负调节因子）开发的功能标记，用于小麦的标记辅助选择
DOI：
10.1016/j.cj.2020.03.003
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发表时间：
2020-05
期刊：
影响因子：
6.6
通讯作者：
Li Junming
中科院分区：
文献类型：
--
作者：
Zhang Wei;Li Huifang;Zhi Liya;Su Qiannan;Liu Jiajia;Ren Xiaoli;Meng Deyuan;Zhang Na;Ji Jun;Zhang Xueyong;Li Junming
克隆了位于六倍体小麦7A、4A、7D染色体上的TaGS3同源基因。对3个TaGS3同源基因的相对表达分析表明，TaGS3-4A和TaGS3-7D在籽粒发育过程中的表达水平均高于TaGS3-7A。遗传证据表明，TaGS3是粒重和粒重的负调控基因。TaGS3-4A共检测到15个多态位点和5个单倍型。开发了两个分子标记来区分这五种单倍型。对260份中国小麦微核心种质的关联分析表明，TaGS3-4A影响千粒重和粒长，HAP-4A-1和HAP-4A-2是提高千粒重和粒长的有利单倍型，在小麦驯化过程中经历了较强的选择。此外，TaGS3-4A和TaGS3-7D的互作对籽粒性状有显著的加性效应，Hap-4A-1/Hap-7D-2是提高TGW和GL的最佳单倍型组合。在1388份来自世界各地的小麦材料中，有利TaGS3单倍型的频率和地理分布为产量相关性状的选择提供了线索。结果表明，TaGS3-4A对TGW和GL有显著影响。分子标记辅助选择HAP-4A-1/2与HAP-7D-2相结合具有提高小麦产量的潜力。
TheTaGS3homoeologous genes (homoeologs) located on chromosomes 7A, 4A, and 7D in hexaploid wheat were cloned. Relative expression analysis of the threeTaGS3homoeologs revealed that the expression levels ofTaGS3-4AandTaGS3-7Din developing grains were higher than that ofTaGS3-7A. Genetic evidence showed thatTaGS3was a negative regulator of grain weight and grain size. Fifteen polymorphic sites and five haplotypes were detected inTaGS3-4A. Two molecular markers were developed to distinguish the five haplotypes. Association analysis using 260 accessions from Chinese wheat mini-core collection (MCC) indicated thatTaGS3-4Aaffected thousand grain weight (TGW) and grain length (GL).HAP-4A-1andHAP-4A-2were favorable haplotypes that increased TGW and GL and had undergone strong selection during domestication of wheat. In addition, interaction of theTaGS3-4AandTaGS3-7Dhomoeologs had significant additive effects on the grain traits.Hap-4A-1/Hap-7D-2was the best haplotype combination in increasing TGW and GL. The frequencies and geographic distributions of favorableTaGS3haplotypes among 1388 wheat accessions from worldwide sources provided clues for selection of yield-related traits. Our findings demonstrated thatTaGS3-4Ahad significant effects on TGW and GL. Marker-assisted selection ofHAP-4A-1/2 combined withHAP-7D-2has potential to increase wheat yields.
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影响因子：
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发表时间：
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影响因子：
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影响因子：
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