A multifactor analysis of fungal and bacterial community structure in the root microbiome of mature Populus deltoides trees.
A multifactor analysis of fungal and bacterial community structure in the root microbiome of mature Populus deltoides trees.
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DOI：
10.1371/journal.pone.0076382
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Schadt CW
中科院分区：
文献类型：
--
作者：
Shakya M;Gottel N;Castro H;Yang ZK;Gunter L;Labbé J;Muchero W;Bonito G;Vilgalys R;Tuskan G;Podar M;Schadt CW
与植物根部相关的细菌和真菌群落对宿主的健康、存活和生长至关重要。然而，对根微生物组和驱动宿主相关微生物群落结构的因素的深入了解仍然难以捉摸，特别是在自然环境中的成熟多年生植物中。本研究以美国黑杨（Populus deltoides）为研究对象，研究了根际和根内土壤细菌和真菌群落的相互关系，以及土壤参数、环境特性对细菌和真菌群落的影响（寄主表型和地上环境），寄主植物基因型（简单重复序列（SSR）标记），季节（春季与秋季）和地理环境（从区域流域到当地河岸带的规模）对微生物群落结构的影响。每棵采样的树木都显示出其相关群落结构的独特方面，具有大量特定于个体树木的操作分类单位（OTU）（细菌> 90%，真菌>60%）。在调查的各种条件下，只有少量OTU是常见的根际（35个细菌和4个真菌）和内圈（1个细菌和1个真菌）微生物组内的所有样品。正如预期的那样，变形菌门和子囊菌门在根群落中占主导地位（>50%），而其他更高层次的系统发育类群（壶菌门，酸杆菌）在根际相比，内圈中的丰度大大减少。方差分配部分解释了基于季节和土壤性质的所有采样根之间微生物组组成的差异（4%至23%）。虽然大多数变化仍然未归因，但我们观察到流域（田纳西州与北卡罗来纳州）和季节（春季与秋季）之间的微生物群存在显着差异。SSR标记清楚地描绘了两个主机种群与TN与NC中采取的样品，但总体主机基因型距离没有显着影响相应的社区，可以从其他测量的效果分开。
Bacterial and fungal communities associated with plant roots are central to the host health, survival and growth. However, a robust understanding of the root-microbiome and the factors that drive host associated microbial community structure have remained elusive, especially in mature perennial plants from natural settings. Here, we investigated relationships of bacterial and fungal communities in the rhizosphere and root endosphere of the riparian tree species Populus deltoides, and the influence of soil parameters, environmental properties (host phenotype and aboveground environmental settings), host plant genotype (Simple Sequence Repeat (SSR) markers), season (Spring vs. Fall) and geographic setting (at scales from regional watersheds to local riparian zones) on microbial community structure. Each of the trees sampled displayed unique aspects to its associated community structure with high numbers of Operational Taxonomic Units (OTUs) specific to an individual trees (bacteria >90%, fungi >60%). Over the diverse conditions surveyed only a small number of OTUs were common to all samples within rhizosphere (35 bacterial and 4 fungal) and endosphere (1 bacterial and 1 fungal) microbiomes. As expected, Proteobacteria and Ascomycota were dominant in root communities (>50%) while other higher-level phylogenetic groups (Chytridiomycota, Acidobacteria) displayed greatly reduced abundance in endosphere compared to the rhizosphere. Variance partitioning partially explained differences in microbiome composition between all sampled roots on the basis of seasonal and soil properties (4% to 23%). While most variation remains unattributed, we observed significant differences in the microbiota between watersheds (Tennessee vs. North Carolina) and seasons (Spring vs. Fall). SSR markers clearly delineated two host populations associated with the samples taken in TN vs. NC, but overall host genotypic distances did not have a significant effect on corresponding communities that could be separated from other measured effects.
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DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
影响因子：
2.5
作者：
Doty, Sharon L.;Oakley, Brian;Staley, James T.
通讯作者：
Staley, James T.
影响因子：
6.1
作者：
Arft, AM;Walker, MD;Wookey, PA
通讯作者：
Wookey, PA
影响因子：
4.4
作者：
Lauber, Christian L.;Hamady, Micah;Fierer, Noah
通讯作者：
Fierer, Noah
影响因子：
2.6
作者：
Danielsen, L.;Thuermer, A.;Meinicke, P.;Buee, M.;Morin, E.;Martin, F.;Pilate, G.;Daniel, R.;Polle, A.;Reich, M.
通讯作者：
Reich, M.