Regulation of pentose utilisation by AraR, but not XlnR, differs in Aspergillus nidulans and Aspergillus niger.
Regulation of pentose utilisation by AraR, but not XlnR, differs in Aspergillus nidulans and Aspergillus niger.
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DOI：
10.1007/s00253-011-3242-2
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发表时间：
2011-07
影响因子：
5
通讯作者：
de Vries, Ronald P.
中科院分区：
文献类型：
--
作者：
Battaglia, Evy;Hansen, Sara Fasmer;Leendertse, Anne;Madrid, Susan;Mulder, Harm;Nikolaev, Igor;de Vries, Ronald P.
丝状真菌是植物多糖降解酶的重要生产者，其在许多工业应用中使用。这些酶由真菌产生，以释放用作碳源的单糖。植物多糖的两种主要成分是l-阿拉伯糖和d-木糖，它们在这些真菌中通过戊糖分解代谢途径（PCP）代谢。在尼日尔曲霉中，从多糖和PCP释放戊糖的调节涉及转录激活因子AraR和XlnR，其也存在于其他曲霉如构巢曲霉中。比较分析表明，AraR对PCP的调控作用在A. nidulans和A.尼日尔，而XlnR对PCP的调节在两种物种中相似。这通过A中araR和xlnR的破坏菌株之间在l-阿拉伯糖上的生长差异来证明。nidulans和A.尼日尔.此外，编码L-阿拉伯糖还原酶（larA），L-阿拉伯糖醇脱氢酶（ladA）和木糖醇脱氢酶（xdhA）的基因的表达谱在这些菌株中不同。这些数据表明，这两个物种的进化变化影响戊糖的利用。这项研究还表明，操纵调节系统，以提高多糖降解酶的生产，可能会得到不同的结果，在不同的工业真菌。本文的在线版本（doi：10.1007/s 00253 -011-3242-2）包含补充材料，可供授权用户使用。
Filamentous fungi are important producers of plant polysaccharide degrading enzymes that are used in many industrial applications. These enzymes are produced by the fungus to liberate monomeric sugars that are used as carbon source. Two of the main components of plant polysaccharides are l-arabinose and d-xylose, which are metabolized through the pentose catabolic pathway (PCP) in these fungi. In Aspergillus niger, the regulation of pentose release from polysaccharides and the PCP involves the transcriptional activators AraR and XlnR, which are also present in other Aspergilli such as Aspergillus nidulans. The comparative analysis revealed that the regulation of the PCP by AraR differs in A. nidulans and A. niger, whereas the regulation of the PCP by XlnR was similar in both species. This was demonstrated by the growth differences on l-arabinose between disruptant strains for araR and xlnR in A. nidulans and A. niger. In addition, the expression profiles of genes encoding l-arabinose reductase (larA), l-arabitol dehydrogenase (ladA) and xylitol dehydrogenase (xdhA) differed in these strains. This data suggests evolutionary changes in these two species that affect pentose utilisation. This study also implies that manipulating regulatory systems to improve the production of polysaccharide degrading enzymes, may give different results in different industrial fungi. The online version of this article (doi:10.1007/s00253-011-3242-2) contains supplementary material, which is available to authorized users.
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