Lipidomic analysis of twenty-seven prostanoids and isoprostanes by liquid chromatography/electrospray tandem mass spectrometry.
Lipidomic analysis of twenty-seven prostanoids and isoprostanes by liquid chromatography/electrospray tandem mass spectrometry.
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DOI：
10.1002/rcm.2697
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发表时间：
2006
影响因子：
2
通讯作者：
Nicolaou, Anna
中科院分区：
文献类型：
--
作者：
Masoodi, Mojgan;Nicolaou, Anna
前列腺素类是许多生理和病理生理过程的有效介质。在用于其定性和定量分析的许多分析方法中，电喷雾串联质谱与液相色谱（ESI-LC-MS/MS）联用提供了一种适用于脂质组学分析的快速、灵敏和通用的系统。我们已经开发了27种介质的ESI-LC-MS/MS分析，包括前列腺素，前列环素，血栓烷，二氢前列腺素和异前列腺素。在1-100 pg/μL浓度范围内，测定呈线性。检测限和定量限分别为0.5-50 pg和2-100 pg，回收率为83- 116%，具体取决于代谢物。该测定可用于分析由各种生物液体和提取物（包括脑、肝、血浆和尿液）产生的前列腺素类化合物，从而促进我们对这些脂质介质在健康和疾病中的作用的理解，以及协助药物开发。
Prostanoids are potent mediators of many physiological and pathophysiological processes. Of the many analytical methodologies used for their qualitative and quantitative analysis, electrospray tandem mass spectrometry coupled to liquid chromatography (ESI-LC-MS/MS) offers a rapid, sensitive and versatile system applicable to lipidomic analyses. We have developed an ESI-LC-MS/MS assay for twenty-seven mediators including prostaglandins, prostacyclines, thromboxanes, dihydroprostaglandins and isoprostanes. The assay was liner over the concentration range 1-100 pg/μL. The limits of detection and quantitation were 0.5-50 pg and 2-100 pg respectively, whilst recoveries were from 83-116 % depending on the metabolite. The assay can be applied to profiling prostanoids produced by a variety of biological fluids and extracts including brain, liver, plasma and urine, facilitating thus our understanding of the role of these lipid mediators in health and disease, as well as assisting in drug development.
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