Molecular characteristics of extended-spectrum β-lactamase-producing Escherichia coli in Riyadh: emergence of CTX-M-15-producing E. coli ST131.
Molecular characteristics of extended-spectrum β-lactamase-producing Escherichia coli in Riyadh: emergence of CTX-M-15-producing E. coli ST131.
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DOI：
10.1186/1476-0711-13-4
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发表时间：
2014-01-07
影响因子：
5.7
通讯作者：
Khubnani H
中科院分区：
文献类型：
--
作者：
Al-Agamy MH;Shibl AM;Hafez MM;Al-Ahdal MN;Memish ZA;Khubnani H
近年来，产超广谱β-内酰胺酶大肠埃希菌（ESBL-EC）的感染率呈上升趋势。本研究的目的是进一步了解和评估利雅得ESBL-EC的发生情况，并采用脉冲场凝胶电泳（PFGE）技术进行ESBL-EC的流行病学调查和ST 131在ESBL-EC中的流行情况。共152个E。2010年9月至2011年6月在利雅得的一家三级医院收集大肠杆菌分离株。采用基因型和表型方法检测ESBLs。PFGE用于确定遗传相关性。采用实时荧光定量PCR检测ST 131和CTX-M样ESBLs。在152株菌株中，表型法检测ESBLs阳性31株。31株ESBL阳性大肠埃希菌中，blaCTX-M-15基因占96.77%（30/31）。大肠杆菌菌株。1株检出blaCTX-M-27基因。31例ESBL-EC中20例（64.5%）为ST 131。PFGE显示了29种不同的脉型。我们的研究证实了ESBLs在大肠埃希菌中的高流行率。大肠埃希菌ESBL基因以CTX-M-15为主。ST 131克隆生产CTX-M-15在我们医院有很大的存在。CTX-M生产者的高流行率不是由于单个克隆的传播。据我们所知，本研究首次报道了沙特大肠杆菌中的CTX-M-15和CTX-M-27 β-内酰胺酶，并检出了ST 131克隆。大肠杆菌分离株。
The prevalence of extended-spectrum β-lactamase-producing Escherichia coli (ESBL-EC) has increased recently. The aim of this study was to further characterise and to assess the occurrence of ESBL-EC in Riyadh, to use pulsed field gel electrophoresis (PFGE) typing to investigate the epidemiology of ESBL-EC and to determine the prevalence of ST131 in ESBL-EC. A total of 152 E. coli isolates were collected at a tertiary hospital in Riyadh from September 2010 to June 2011. Genotypic and phenotypic methods were used to characterise ESBLs. PFGE was used to determine genetic relatedness. Detection of ST131 and CTX-M-like ESBLs was performed using real-time PCR. Of 152 strains, 31 were positive for ESBLs by phenotypic methods. The blaCTX-M-15 gene was highly prevalent (30/31 strains, 96.77%) among the 31 ESBL-positive E. coli strains. The blaCTX-M-27 gene was detected in one strain. Twenty (64.5%) out of 31 of ESBL-EC were ST131. PFGE revealed 29 different pulsotypes. Our study documented the high prevalence of ESBLs in E. coli isolates, with CTX-M-15 as the predominant ESBL gene. ST131 clone producing CTX-M-15 has a major presence in our hospital. The high prevalence of CTX-M producers was not due to the spread of a single clone. To the best of our knowledge, this study represents the first report of CTX-M-15 and CTX-M-27 β-lactamases and the detection of the ST131 clone in Saudi E. coli isolates.
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