Immune-inflammation gene signatures in endometriosis patients.
Immune-inflammation gene signatures in endometriosis patients.
复制标题
DOI：
10.1016/j.fertnstert.2016.07.005
复制
发表时间：
2016-11
影响因子：
6.7
通讯作者：
Tayade C
中科院分区：
文献类型：
--
作者：
Ahn SH;Khalaj K;Young SL;Lessey BA;Koti M;Tayade C
确定子宫内膜异位症病变的分子特征是否包含炎症和免疫功能障碍的信息性指标，这可能有助于更好地了解免疫功能障碍和炎症之间的相互作用及其对子宫内膜异位症发病机制的贡献。使用Nanostring nCounter GX Human Immunology V2平台进行免疫和炎症转录组学分析（579个人免疫和炎症相关基因和15个管家基因）。学术大学和教学医院。没有。患有不孕症的III-IV期子宫内膜异位症患者（n = 8）和接受输卵管结扎术的无生育疾病对照女性（n = 8）。所有参与者的月经期与分泌期相匹配。患者异位增生病变和匹配的在位子宫内膜的免疫和炎症转录组学定量。对照组为正常生育妇女子宫内膜。我们的研究结果显示，异位病变作为分子上不同的实体相比，在位内膜和对照组子宫内膜; 396的579个筛选免疫和炎症相关基因在异位组织与对照组子宫内膜相比，有显着差异。最重要的是，与对照子宫内膜相比，患者的在位子宫内膜显示出独特的分子谱（91/579基因显著不同），特别是参与细胞凋亡和蜕膜化调节的基因。我们研究了子宫内膜异位症患者免疫炎症基因的差异表达，并与正常生育妇女比较，显示了患者在位子宫内膜的分子差异。
To determine if the molecular profiles of endometriotic lesions contain informative measures of inflammation and immune dysfunction that may contribute to better understanding of the interplay between immune dysfunction and inflammation and their contribution to endometriosis pathogenesis. Immune and inflammation transcriptomic analysis with the use of the Nanostring nCounter GX Human Immunology V2 platform (579 human immune and inflammation–related genes and 15 housekeeping genes). Academic university and teaching hospital. None. Stage III–IV endometriosis patients with infertility (n = 8) and fertile disease-free control women undergoing tubal ligation (n = 8). Menstrual stage was matched to secretory phase in all participants. Immune and inflammation transcriptomics quantification from ectopic endometriotic lesions and matched eutopic endometrium from patients. Endometria of fertile women served as control subjects. Our results displayed endometriotic lesions as molecularly distinct entities compared with eutopic endometrium and endometrium of control samples; 396 out of 579 screened immune and inflammation–related genes were significantly different in ectopic tissues compared with control endometrium. Most importantly, eutopic endometrium of the patients displayed a unique molecular profile compared with the control endometrium (91/579 genes were significantly different), particularly of genes involved in regulation of cell apoptosis and decidualization. We characterize differential expression of immune-inflammation genes in endometriosis patients, and show molecular distinction of eutopic endometrium of patients compared with control fertile women.
登录
查看更多内容
影响因子：
64.5
作者：
Han SJ;Jung SY;Wu SP;Hawkins SM;Park MJ;Kyo S;Qin J;Lydon JP;Tsai SY;Tsai MJ;DeMayo FJ;O'Malley BW
通讯作者：
O'Malley BW
DOI：
10.12659/msm.881707
发表时间：
2011-04
期刊：
Medical science monitor : international medical journal of experimental and clinical research
影响因子：
--
作者：
Liu H;Lang JH
通讯作者：
Lang JH
影响因子：
4.4
作者：
Deng, AM;Chen, SX;Krensky, AM
通讯作者：
Krensky, AM
影响因子：
6.7
作者：
Lebovic, DI;Mueller, MD;Taylor, RN
通讯作者：
Taylor, RN
DOI：
10.4049/jimmunol.1501138
发表时间：
2015-09-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Ahn SH;Edwards AK;Singh SS;Young SL;Lessey BA;Tayade C
通讯作者：
Tayade C