Genetic Analysis of Babesia Isolates from Cattle with Clinical Babesiosis in Sri Lanka
Genetic Analysis of Babesia Isolates from Cattle with Clinical Babesiosis in Sri Lanka
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斯里兰卡临床巴贝虫病牛巴贝虫分离株的遗传分析
DOI：
10.1128/jcm.00895-18
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发表时间：
2018
影响因子：
9.4
通讯作者：
Silva Seekku
中科院分区：
文献类型：
--
作者：
Sivakumar Thillaiampalam;Tuvshintulga Bumduuren;Zhyldyz Atambekova;Kothalawala Hemal;Yapa Palitha Rohana;Kanagaratnam Ratnam;Vimalakumar Singarayar Caniciyas;Abeysekera Thuduwege Sanath;Weerasingha Amitha Sampath;Yamagishi Junya;Igarashi Ikuo;Silva Seekku
牛巴贝斯虫病是养牛业的严重威胁。我们从斯里兰卡Badulla（n= 8），Jaffna（n= 3）和Kilinochchi（n= 2）地区的13头临床巴贝斯虫病牛中制备了血液DNA样本。这些DNA样本在巴贝斯克线虫（n= 9）、二联巴贝斯克线虫（n= 9）和巴贝斯克线虫（n= 1）特异性PCR检测中检测为阳性。12头牛B阳性。牛和/或B。二联。1头奶牛对测试的巴贝虫属呈阴性，但在显微镜检查中对巴贝虫呈阳性; 18 S rRNA和细胞色素氧化酶亚基III基因序列的系统发育位置表明，该奶牛感染了巴贝虫属Mymensingh，这是最近从孟加拉国的健康奶牛中报告的。然后，我们开发了一种新的巴贝虫属Mymensingh特异性PCR检测，并获得了另一个样品的阳性结果。B.卵特异性PCR结果表明，该动物未感染B. ovatabut与Babelussp. Hue-1，这是最近在越南无症状牛报道。由于奶牛同时感染了巴氏杆菌，因此巴氏杆菌的毒力尚不清楚。牛肝菌B.二联。然而，麦门星巴贝斯虫可能导致严重的临床巴贝斯虫病，因为它是在临床病例中检测到的唯一巴贝斯虫物种。目前的研究揭示了两种以前在斯里兰卡没有报道的牛巴贝虫物种的存在，以及第一例由巴贝虫物种而不是B引起的严重牛巴贝虫病。bovis，牛湾（B. bigemina和babeliadivergens。
Bovine babesiosis is a serious threat to the cattle industry. We prepared blood DNA samples from 13 cattle with clinical babesiosis from the Badulla (n= 8), Jaffna (n= 3), and Kilinochchi (n= 2) districts in Sri Lanka. These DNA samples tested positive in PCR assays specific for Babesiabovis(n= 9), Babesia bigemina (n= 9), and Babesiaovata(n= 1). Twelve cattle were positive forB. bovisand/or B. bigemina. One cow was negative for the tested Babesia species but was positive for Babesia on microscopic examination; the phylogenetic positions of 18S rRNA and cytochrome oxidase subunit III gene sequences suggested that the cow was infected with Babesia sp. Mymensingh, which was recently reported from a healthy cow in Bangladesh. We then developed a novel Babesia sp. Mymensingh-specific PCR assay and obtained positive results for one other sample. Analysis of gene sequences from the cow with positiveB. ovata-specific PCR results demonstrated that the animal was infected not withB. ovatabut with Babesia sp. Hue-1, which was recently reported from asymptomatic cattle in Vietnam. The virulence of Babesia sp. Hue-1 is unclear, as the cow was coinfected withB. bovisand B. bigemina. However, Babesia sp. Mymensingh probably causes severe clinical babesiosis, as it was the sole Babesia species detected in a clinical case. The present study revealed the presence of two bovine Babesia species not previously reported in Sri Lanka, plus the first case of severe bovine babesiosis caused by a Babesia species other thanB. bovis, B. bigemina, and Babesia divergens.
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斯里兰卡和澳大利亚牛巴贝斯虫株在斯里兰卡进口牛疫苗接种中的比较
影响因子：
1.7
作者：
D. Weilgama;W. Jorgensen;R. J. Dalgliesh;M. Navaratne;C. Weerasinghe
通讯作者：
C. Weerasinghe
DOI：
10.4038/jnsfsr.v25i1.5016
发表时间：
1997
影响因子：
0.5
作者：
H. Abeygunawardena;D. Rathnayaka;W. Jayathilaka
通讯作者：
W. Jayathilaka
影响因子：
1.2
作者：
Ota, Naomi;Mizuno, Daisuke;Yokoyama, Naoaki
通讯作者：
Yokoyama, Naoaki
影响因子：
1.2
作者：
Ybanez, Adrian Patalinghug;Sivakumar, Thillaiampalam;Inokuma, Hisashi
通讯作者：
Inokuma, Hisashi
东南亚猕猴的生物多样性
DOI：
--
发表时间：
2005
期刊：
Nat. Hist. J. Chula Univ. Suppl 1
影响因子：
--
作者：
Hamada;Y
通讯作者：
Y