Microscale impeller pump for recirculating flow in organs-on-chip and microreactors.
Microscale impeller pump for recirculating flow in organs-on-chip and microreactors.
复制标题
DOI：
10.1039/d1lc01081f
复制
发表时间：
2022-02-01
期刊：
影响因子：
6.1
通讯作者：
Pompano RR
中科院分区：
文献类型：
--
作者：
Cook SR;Musgrove HB;Throckmorton AL;Pompano RR
流体流量是微流体和器官芯片技术的组成部分，理想地提供了仿生的液体，细胞和营养交换，以及当前的物理或病理剪切应力，许多在生物型流动速度上积极刺激流体的泵与在细胞培养物中的使用相关，以使许多型号构成了许多构造，许多泵都能构成这些问题。 user-friendly impeller pump that uses a 3D-printed device and impeller to recirculate fluid and cells on-chip. Impeller rotation was driven by a rotating magnetic field generated by magnets mounted on a computer fan; this pump platform required no tubing connections and could accommodate up to 36 devices at once in a standard cell culture incubator. A computational model was used to predict shear stress, velocity, and changes in pressure throughout the device. The impeller pump通过调谐通道和入口尺寸和叶轮的旋转速度控制的仿生液速度（50-6400μm/s）与计算剪切范围的速度相当。 Jurkat T细胞24小时，否对细胞生存能力的影响，显示叶轮泵在未来对白细胞再循环的可行性。
Fluid flow is an integral part of microfluidic and organ-on-chip technology, ideally providing biomimetic fluid, cell, and nutrient exchange as well as physiological or pathological shear stress. Currently, many of the pumps that actively perfuse fluid at biomimetic flow rates are incompatible with use inside cell culture incubators, require many tubing connections, or are too large to run many devices in a confined space. To address these issues, we developed a user-friendly impeller pump that uses a 3D-printed device and impeller to recirculate fluid and cells on-chip. Impeller rotation was driven by a rotating magnetic field generated by magnets mounted on a computer fan; this pump platform required no tubing connections and could accommodate up to 36 devices at once in a standard cell culture incubator. A computational model was used to predict shear stress, velocity, and changes in pressure throughout the device. The impeller pump generated biomimetic fluid velocities (50-6400 μm/s) controllable by tuning channel and inlet dimensions and the rotational speed of the impeller, which were comparable to the order of magnitude of the velocities predicted by the computational model. Predicted shear stress was in the physiological range throughout the microchannel and over the majority of the impeller. The impeller pump successfully recirculated primary murine splenocytes for 1 hr and Jurkat T cells for 24 hr with no impact on cell viability, showing the impeller pump’s feasibility for white blood cell recirculation on-chip. In the future, we envision that this pump will be integrated into single- or multi-tissue platforms to study communication between organs.
登录
查看更多内容
影响因子：
4.6
作者：
Buerck, James P.;Burke, Dustin K.;O'Rear, Edgar A.
通讯作者：
O'Rear, Edgar A.
DOI：
10.1021/acs.langmuir.1c00625
发表时间：
2021-06-22
期刊：
Langmuir : the ACS journal of surfaces and colloids
影响因子：
--
作者：
Catterton MA;Montalbine AN;Pompano RR
通讯作者：
Pompano RR
影响因子：
4.6
作者：
Edington CD;Chen WLK;Geishecker E;Kassis T;Soenksen LR;Bhushan BM;Freake D;Kirschner J;Maass C;Tsamandouras N;Valdez J;Cook CD;Parent T;Snyder S;Yu J;Suter E;Shockley M;Velazquez J;Velazquez JJ;Stockdale L;Papps JP;Lee I;Vann N;Gamboa M;LaBarge ME;Zhong Z;Wang X;Boyer LA;Lauffenburger DA;Carrier RL;Communal C;Tannenbaum SR;Stokes CL;Hughes DJ;Rohatgi G;Trumper DL;Cirit M;Griffith LG
通讯作者：
Griffith LG
影响因子：
3.9
作者：
通讯作者：
--
影响因子：
6.1
作者：
Au AK;Bhattacharjee N;Horowitz LF;Chang TC;Folch A
通讯作者：
Folch A