Ectopic expression of a cytochrome P450 monooxygenase gene PtCYP714A3 from Populus trichocarpa reduces shoot growth and improves tolerance to salt stress in transgenic rice.
Ectopic expression of a cytochrome P450 monooxygenase gene PtCYP714A3 from Populus trichocarpa reduces shoot growth and improves tolerance to salt stress in transgenic rice.
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DOI：
10.1111/pbi.12544
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发表时间：
2016-09
影响因子：
13.8
通讯作者：
Zhang H
中科院分区：
文献类型：
--
作者：
Wang C;Yang Y;Wang H;Ran X;Li B;Zhang J;Zhang H
在拟南芥和水稻中，细胞色素P450（P450）714蛋白家族代表了一组独特的单加氧酶，其通过赤霉素失活在植物发育中作为芽特异性调节剂发挥作用。在这里，我们报告PtCYP 714 A3，OsCYP 714 D1/Eui同源物从胡杨的功能特性。PtCYP 714 A3基因在杨树形成层-韧皮部组织中表达量最高，且受盐胁迫和渗透胁迫的诱导。亚细胞定位分析表明PtCYP 714 A3-YFP融合蛋白靶向内质网（ER）。PtCYP 714 A3在水稻eui突变体中的表达可以挽救其过度芽生长表型。PtCYP 714 A3在水稻中的异位表达导致半矮化表型，具有促进的分蘖和减小的种子大小。表现出PtCYP 714 A3显著表达的转基因株系也比野生型（WT）植物积累更低的GA水平。在这些转基因植株中，一些GA生物合成基因的表达被显著抑制。此外，转基因水稻表现出更强的耐盐性，并保持更多的Na+在盐胁迫下的地上部和根组织。这些结果不仅表明PtCYP 714 A3在水稻幼苗对盐胁迫的反应中起着重要作用，而且为耐盐作物的基因工程提供了分子基础。
In Arabidopsis thaliana and Oryza sativa, the cytochrome P450 (CYP) 714 protein family represents a unique group of CYP monooxygenase, which functions as a shoot‐specific regulator in plant development through gibberellin deactivation. Here, we report the functional characterizations of PtCYP714A3, an OsCYP714D1/Eui homologue from Populus trichocarpa. PtCYP714A3 was ubiquitously expressed with the highest transcript level in cambium–phloem tissues, and was greatly induced by salt and osmotic stress in poplar. Subcellular localization analyses indicated that PtCYP714A3‐YFP fusion protein was targeted to endoplasmic reticulum (ER). Expression of PtCYP714A3 in the rice eui mutant could rescue its excessive‐shoot‐growth phenotype. Ectopic expression of PtCYP714A3 in rice led to semi‐dwarfed phenotype with promoted tillering and reduced seed size. Transgenic lines which showed significant expression of PtCYP714A3 also accumulated lower GA level than did the wild‐type (WT) plants. The expression of some GA biosynthesis genes was significantly suppressed in these transgenic plants. Furthermore, transgenic rice plants exhibited enhanced tolerance to salt and maintained more Na+ in both shoot and root tissues under salinity stress. All these results not only suggest a crucial role of PtCYP714A3 in shoot responses to salt toxicity in rice, but also provide a molecular basis for genetic engineering of salt‐tolerant crops.
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影响因子：
5.1
作者：
Igarashi, Y;Yoshiba, Y;Shinozaki, K
通讯作者：
Shinozaki, K
影响因子：
7.3
作者：
Huang, Jin-Guang;Yang, Mei;Zheng, Cheng-Chao
通讯作者：
Zheng, Cheng-Chao
影响因子：
7.2
作者：
Liu, W;Xu, ZH;Xue, HW
通讯作者：
Xue, HW
影响因子：
4.9
作者：
Luo, AD;Qian, Q;Chu, CC
通讯作者：
Chu, CC
影响因子：
6.4
作者：
MURASHIGE, T;SKOOG, F
通讯作者：
SKOOG, F