The surface charge of isolated toad bladder epithelial cells. Mobility, effect of pH and divalent ions.
The surface charge of isolated toad bladder epithelial cells. Mobility, effect of pH and divalent ions.
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DOI：
10.1085/jgp.49.3.501
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发表时间：
1966-01
期刊：
影响因子：
--
通讯作者：
Hays RM
中科院分区：
文献类型：
--
作者：
Lipman KM;Dodelson R;Hays RM
通过细胞电泳的显微镜方法测定了从蟾蜍膀胱中分离的上皮细胞的表面电荷。这些电池具有净负电荷，在 pH 7.3 时，净表面电荷密度为每平方微米 3.6 x 104 个电子电荷。对碱性 pH 范围内的净表面电荷的估计表明 (a) 40 A 数量级的平均距离分隔带负电的基团，以及 (b) 剪切的电泳表面上存在氨基和酸基团。氰酸盐处理细胞后迁移率显着增加，表明大部分氨基是赖氨酸的ε-氨基。鉴于钙和其他二价离子对细胞通透性和细胞粘附的已知影响，通过电泳技术测定钙和镁与细胞表面的结合程度。在存在钙或镁的情况下，迁移率显着降低，表明这些离子被表面基团结合。当 pH 从 7.3 降至 5.2 时，钙结合显着减少，这一观察结果与钙离子和氢离子之间对共同受体位点的竞争一致。
The surface charge of epithelial cells isolated from the toad bladder has been determined by the microscope method of cell electrophoresis. The cells possess a net negative charge, and a net surface charge density of 3.6 x 104 electronic charges per square micron at pH 7.3. Estimates of net surface charge over the alkaline pH range indicate (a) that an average distance of the order of 40 A separates the negatively charged groups, and (b) that amino as well as acid groups are present at the electrophoretic surface of shear. A significant increase in mobility following cyanate treatment of the cells suggests that a large proportion of the amino groups are the ε-amino groups of lysine. In view of the known effects of calcium and other divalent ions on cell permeability and cell adhesion, the extent of binding of calcium and magnesium to the cell surface was determined by the electrophoretic technique. Mobility was significantly decreased in the presence of calcium or magnesium, indicating that these ions are bound by surface groups. When the pH was lowered from 7.3 to 5.2, calcium binding was markedly decreased, an observation consistent with competition between calcium and hydrogen ions for a common receptor site.
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影响因子：
2.9
作者：
MILDVAN, AS;COHN, M
通讯作者：
COHN, M
影响因子：
4.1
作者：
IRONS, LI;PERKINS, DJ
通讯作者：
PERKINS, DJ
影响因子：
7.8
作者：
CHOI, JK
通讯作者：
CHOI, JK
影响因子：
15
作者：
GURD, FRN;GOODMAN, DS
通讯作者：
GOODMAN, DS
影响因子：
64.8
作者：
MORRILL, GA;KABACK, HR;ROBBINS, E
通讯作者：
ROBBINS, E