Loss of intestinal nuclei and intestinal integrity in aging C. elegans.
Loss of intestinal nuclei and intestinal integrity in aging C. elegans.
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DOI：
10.1111/j.1474-9726.2011.00713.x
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发表时间：
2011-08
期刊：
影响因子：
7.8
通讯作者：
Melov S
中科院分区：
文献类型：
--
作者：
McGee MD;Weber D;Day N;Vitelli C;Crippen D;Herndon LA;Hall DH;Melov S
蛔虫C.秀丽隐杆线虫被广泛用作衰老模型，已鉴定出数百种调节衰老的基因。959个细胞组成的成体的发育和体型已经被很好地描述和建立了超过25年。然而，这种光学透明动物随年龄的形态变化还不太清楚，只有少数研究调查衰老的病理生物学。已经通过各种传统的相对低通量的方法观察到肌肉、神经元、肠和卵黄颗粒、核结构、尾核和生殖系中与年龄相关的变化。我们在这里报告了一些新的方法来研究衰老的病理生物学。优美的我们将连续切片组织的组织学染色、透射电子显微镜和共聚焦显微镜与3-D体积重建相结合，并表征了不同年龄的多个野生型个体的年龄相关形态学变化。这使我们能够在C. Elegans肠的变化，包括临界核的丢失，肠微绒毛的降解，肠的大小，形状和细胞质内容物的变化，以及由于摄入细菌而改变的形态。我们从不同年龄的多个个体中创建的组织和细胞成分的三维模型代表了一种独特的资源，可以证明多细胞生物体的全球异质性。
The roundworm C. elegans is widely used as an aging model, with hundreds of genes identified that modulate aging. The development and bodyplan of the 959 cells comprising the adult have been well described and established for more than 25 years. However, morphological changes with age in this optically transparent animal are less well understood, with only a handful of studies investigating the pathobiology of aging. Age related changes in muscle, neurons, intestine and yolk granules, nuclear architecture, tail nuclei, and the germline have been observed via a variety of traditional relatively low-throughput methods. We report here a number of novel approaches to study the pathobiology of aging C. elegans. We combined histological staining of serial-sectioned tissues, transmission electron microscopy, and confocal microscopy with 3-D volumetric reconstructions, and characterized age-related morphological changes of multiple wild-type individuals at different ages. This enabled us to identify several novel pathologies with age in the C. elegans intestine, including loss of critical nuclei, degradation of intestinal microvilli, changes in the size, shape, and cytoplasmic contents of the intestine, and altered morphologies due to ingested bacteria. The three-dimensional models we have created of tissues and cellular components from multiple individuals of different ages, represent a unique resource to demonstrate global heterogeneity of a multi-cellular organism.
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影响因子：
56.9
作者：
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通讯作者：
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