Comparative anatomy of the primate major histocompatibility complex DR subregion: evidence for combinations of DRB genes conserved across species.
Comparative anatomy of the primate major histocompatibility complex DR subregion: evidence for combinations of DRB genes conserved across species.
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灵长类主要组织相容性复合体 DR 亚区的比较解剖学：跨物种保守的 DRB 基因组合的证据。
DOI：
10.1016/s0888-7543(05)80224-1
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发表时间：
1992
期刊：
影响因子：
4.4
通讯作者：
Klein,J
中科院分区：
文献类型：
--
作者：
Kasahara,M;Klein,D;Vincek,V;Sarapata,DE;Klein,J
人类主要组织相容性复合体（HLA）的II类区由三个主要的亚区组成，分别命名为DR、DQ和DP。为了深入了解DR单倍型的进化和稳定性，我们从一只西部低地大猩猩的DR亚区（Gogo-DR）中分离了63个粘粒克隆。所有这些粘粒克隆，但一个被发现落入两个集群。较大的簇A由41个重叠的粘粒克隆定义，包含由外显子4至6组成的DRB基因片段和4个DRB基因，命名为Gogo-DRB 6、Gogo-DRB 5 *01、Gogo-DRB 8和Gogo-DRB 3 *01。该簇的总长度为180 kb。第二个簇，B，包括一个145 kb的连续DNA片段，由21个重叠的粘粒克隆组成。簇B包含3个DRB基因，命名为Gogo-DRB 1 *08、Gogo-DRB 2和Gogo-DRB 3 *02。一个含有aDRB假基因的粘粒克隆（WP 1 -9）不能与A簇或B簇连锁。簇A和簇B的结构与已知的HLA-DR单倍型的结构均不相同。然而，两个大猩猩DRB基因，Gogo-DRB 6和Gogo-DRB 5 *01，其人类对应物在HLA-DR 2单倍型中连锁，被发现在簇A中彼此相邻。B群中Gogo-DRB基因的排列与HLA-DR 3/DR 5/DR 6单倍型相似，与HLA-DR 8单倍型相似，推测为大猩猩DR 8单倍型。这些结果表明，某些组合ofDRB基因已被维持，因为时间之前的分歧，人类和大猩猩血统超过500万年前。
The class II region of the human major histocompatibility complex (HLA) is made up of three major subregions designatedDR, DQ, andDP. With the aim of gaining an insight into the evolution and stability ofDRhaplotypes, a total of 63 cosmid clones were isolated from theDRsubregion (Gogo-DR) of a western lowland gorilla. All but one of these cosmid clones were found to fall into two clusters. The larger cluster, A, was defined by 41 overlapping cosmid clones and contained aDRBgene segment made up of exons 4 through 6 and fourDRBgenes, designatedGogo-DRB6, Gogo-DRB5*01, Gogo-DRB8, andGogo-DRB3*01. The total length of this cluster was ∼ 180 kb. The second cluster, B, encompassed a contiguous DNA stretch of ∼ 145 kb and was composed of 21 overlapping cosmid clones. Cluster B contained threeDRBgenes, designatedGogo-DRB1*08, Gogo-DRB2, andGogo-DRB3*02. One cosmid clone (WP1-9) containing aDRBpseudogene could not be linked to either cluster A or B. Neither the organization of cluster A nor that of cluster B was identical to that of knownHLA-DRhaplotypes. However, two gorillaDRBgenes,Gogo-DRB6andGogo-DRB5*01, the human counterparts of which are linked in theHLA-DR2haplotype, were found to be located next to each other in cluster A. The arrangement of theGogo-DRBgenes in cluster B, which is presumed to be the gorillaDR8haplotype, was similar to that ofHLA-DR3/DR5/DR6haplotypes and to that of the presumed ancestralHLA-DR8haplotype. These results demonstrate that certain combinations ofDRBgenes have been maintained since the time before the divergence of human and gorilla lineages more than 5 million years ago.
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