Dynamic Dissection of Dynein and Kinesin-1 Cooperatively Mediated Intercellular Transport of Porcine Epidemic Diarrhea Coronavirus along Microtubule Using Single Virus Tracking.
Dynamic Dissection of Dynein and Kinesin-1 Cooperatively Mediated Intercellular Transport of Porcine Epidemic Diarrhea Coronavirus along Microtubule Using Single Virus Tracking.
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使用单病毒追踪动态解剖动力蛋白和驱动蛋白-1 协同介导的猪流行性腹泻冠状病毒沿微管的细胞间运输
DOI：
10.1080/21505594.2021.1878748
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发表时间：
2021-12
期刊：
影响因子：
5.2
通讯作者：
Liu F
中科院分区：
文献类型：
--
作者：
Hou W;Kang W;Li Y;Shan Y;Wang S;Liu F
ABSTRACT It is now clear that the intercellular transport on microtubules by dynein and kinesin-1 motors has an important role in the replication and spread of many viruses. Porcine epidemic diarrhea virus (PEDV) is an enveloped, single-stranded RNA virus of the Coronavirus family, which can infect swine of all ages and cause severe economic losses in the swine industry. Elucidating the molecular mechanisms of the intercellular transport of PEDV through microtubule, dynein and kinesin-1 will be crucial for understanding its pathogenesis. Here, we demonstrate that microtubule, dynein, and kinesin-1 are involved in PEDV infection and can influence PEDV fusion and accumulation in the perinuclear region but cannot affect PEDV attachment or internalization. Furthermore, we adopted a single-virus tracking technique to dynamically observe PEDV intracellular transport with five different types: unidirectional movement toward microtubule plus ends; unidirectional movement toward microtubule minus ends; bidirectional movement along the same microtubule; bidirectional movement along different microtubules and motionless state. Among these types, the functions of dynein and kinesin-1 in PEDV intercellular transport were further analyzed by single-virus tracking and found that dynein and kinesin-1 mainly transport PEDV to the minus and plus ends of the microtubules, respectively; meanwhile, they also can transport PEDV to the opposite ends of the microtubules different from their conventional transport directions and also coordinate the bidirectional movement of PEDV along the same or different microtubules through their cooperation. These results provided deep insights and references to understand the pathogenesis of PEDV as well as to develop vaccines and treatments.
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Lee C
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Goychuk I;Kharchenko VO;Metzler R
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Metzler R
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Cooper, John A
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Malikov, Viacheslav;da Silva, Eveline Santos;Jovasevic, Vladimir;Bennett, Geoffrey;Vieira, Daniel A. de Souza Aranha;Schulte, Bianca;Diaz-Griffero, Felipe;Walsh, Derek;Naghavi, Mojgan H.
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Naghavi, Mojgan H.
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通讯作者：
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