Differential responses of Mimusops elengi and Manilkara zapota seeds and embryos to cryopreservation
Differential responses of Mimusops elengi and Manilkara zapota seeds and embryos to cryopreservation
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Mimusops elengi 和 Manilkara zapota 种子和胚胎对冷冻保存的差异反应
DOI：
10.1007/s11627-013-9562-4
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发表时间：
2013-10
期刊：
影响因子：
--
通讯作者：
Song Songquan
中科院分区：
文献类型：
--
作者：
Wen Bin;Wang Xiaofeng;Tan Yunhong;Song Songquan
几种脱水方案进行了评估，他们的能力，冷冻保存完整的种子和切下的胚轴Mimusops elengi和Manilkara zapota（人心果科）。耐冷性在种间和种内均存在变异。zapota的胚轴比M. elengi，并表现出较高的耐干燥性，较高的解冻后存活和发育，以及更广泛的水分含量范围的超低温保存。最高发育率分别为94%和27%。zapota和M. elengi，分别。这两个物种的完整种子耐受干燥到低水分水平，但对液氮暴露敏感，冷冻保存的种子未能发芽。从冷冻保存的种子中切下的发育中的胚的评估与子叶和胚芽的非生活力和发芽失败有关。其他结构的存活率各不相同：大多数下胚轴和胚根（M. elengi和M. zapota）是可行的。下胚轴和子叶之间不同的耐低温性是低温保存失败的关键原因，导致难以开发用于此类中间含油种子的方案。
Several dehydration protocols were evaluated for their ability to cryopreserve intact seeds and excised embryonic axes of Mimusops elengi and Manilkara zapota (Sapotaceae). Both interspecific and intraspecific variations in cryotolerance were found. M. zapota embryonic axes were more tolerant of cryopreservation than those of M. elengi, and showed higher desiccation tolerance, higher post-thawing survival and development, and a much wider range of moisture contents for cryopreservation. Maximum development rates were 94% and 27% for M. zapota and M. elengi, respectively. Intact seeds of both species tolerated desiccation to low moisture levels, but were sensitive to liquid nitrogen exposure, and cryopreserved seeds failed to germinate. Assessment of developing embryos excised from cryopreserved seeds associated nonviability of cotyledons and plumules with germination failure. Other structures survived at variable rates; most hypocotyls and radicles (up to 76% and 98% for M. elengi and M. zapota, respectively) were viable. The different cryotolerance between hypocotyls and cotyledons is a critical cause for failure in cryopreservation, contributing to the difficulty in developing protocols for such intermediate oily seeds.
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DOI：
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发表时间：
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期刊：
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影响因子：
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--
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DOI：
--
发表时间：
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期刊：
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