Repertoire of BALB/c Mice Natural Anti-Carbohydrate Antibodies: Mice vs. Humans Difference, and Otherness of Individual Animals.
Repertoire of BALB/c Mice Natural Anti-Carbohydrate Antibodies: Mice vs. Humans Difference, and Otherness of Individual Animals.
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DOI：
10.3389/fimmu.2017.01449
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发表时间：
2017
影响因子：
7.3
通讯作者：
Mañez R
中科院分区：
文献类型：
--
作者：
Bello-Gil D;Khasbiullina N;Shilova N;Bovin N;Mañez R
用作研究人类疾病模型的小鼠最常见的遗传背景之一是近交 BALB/c 品系。这项工作旨在通过印刷聚糖阵列技术表征 20 只 BALB/c 小鼠血清中存在的天然抗碳水化合物抗体的模式，并将其与人类天然抗碳水化合物抗体的结合特异性进行比较。 BALB/c 小鼠血清中的天然抗体 (NAb) 与 419 种不同碳水化合物结构库中的 71 种聚糖相互作用。然而，这些聚糖中只有七种被所有研究动物的血清识别，而其他五种聚糖被至少 80% 的小鼠识别。 BALB/c 小鼠循环抗体主要识别的 12 种聚糖的模式与人类天然抗碳水化合物抗体观察到的模式显着不同。当打算使用小鼠模型来研究生物医学研究中的 NAb 时，应考虑到个体近交小鼠之间以及小鼠与人类之间缺乏相同的天然抗碳水化合物抗体库。
One of the most common genetic backgrounds for mice used as a model to investigate human diseases is the inbred BALB/c strain. This work is aimed to characterize the pattern of natural anti-carbohydrate antibodies present in the serum of 20 BALB/c mice by printed glycan array technology and to compare their binding specificities with that of human natural anti-carbohydrate antibodies. Natural antibodies (NAbs) from the serum of BALB/c mice interacted with 71 glycans from a library of 419 different carbohydrate structures. However, only seven of these glycans were recognized by the serum of all the animals studied, and other five glycans by at least 80% of mice. The pattern of the 12 glycans mostly recognized by the circulating antibodies of BALB/c mice differed significantly from that observed with natural anti-carbohydrate antibodies in humans. This lack of identical repertoires of natural anti-carbohydrate antibodies between individual inbred mice, and between mice and humans, should be taken into consideration when mouse models are intended to be used for investigation of NAbs in biomedical research.
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影响因子：
5.2
作者：
Savage HP;Baumgarth N
通讯作者：
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作者：
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作者：
Richter, D;Incani, RN;Harn, DA
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