Prevalent RNA sequences of mitochondrial origin in sea urchin embryos.

Prevalent RNA sequences of mitochondrial origin in sea urchin embryos.
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海胆胚胎中线粒体起源的普遍 RNA 序列。

DOI:
10.1016/0012-1606(82)90202-0
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发表时间:
1982
影响因子:
2.7
通讯作者:
Raff,RA
Raff,RA
中科院分区:
生物学3区
文献类型:
--
作者:
Wells,DE;Bruskin,AM;O'Brochta,DA;Raff,RA

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分离出了一组cDNA克隆,并与紫红圆海胆(strongylocentrrotus purpuratus)卵和胚胎中常见的rna杂交。克隆人分为两类。a类序列与一系列的4到7个转录本杂交,这些转录本在卵子和胚胎中保持不变。b类序列几乎完全杂交到一个1.5 kb的转录本,在胚胎发育期间增加了流行率。这两类克隆在线粒体基因组的不同区域杂交。a类序列在进化过程中是非常保守的,它们与xenopus线粒体DNA限制性内切片段的杂交模式表明它们代表线粒体核糖体RNA序列。b类序列代表一种功能未知的非保守线粒体RNA。这些RNA序列是在“线粒体后”上清液中发现的,这些上清液是由鸡蛋和蔗糖梯度中的mRNPs等沉积物制备的。
A set of cDNA clones was isolated which hybridize with RNAs very prevalent in eggs and embryos of the sea urchin,Strongylocentrotus purpuratus. The clones fall into two classes. The A-class sequences hybridize to a series of four to seven transcripts which remain constant in prevalence in eggs and embryos. The B-class sequences hybridize almost exclusively to a single 1.5-kb transcript which increases in prevalence during embryonic development. Clones of both classes hybridize to different regions of the mitochondrial genome. The A-class sequences are very conserved in evolution, and their pattern of hybridization with restriction fragments ofXenopusmitochondrial DNA indicates that they represent mitochondrial ribosomal RNA sequences. The B-class sequences represent an unconserved mitochondrial RNA of unknown function. These RNA sequences are found in “postmitochondrial” supernatants prepared from eggs and sediment like mRNPs in sucrose gradients.
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发表时间: 1979
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