Three-dimensional digital microfluidic manipulation of droplets in oil medium.
Three-dimensional digital microfluidic manipulation of droplets in oil medium.
复制标题
DOI：
10.1038/srep10685
复制
发表时间：
2015-06-02
影响因子：
4.6
通讯作者：
Lee SJ
中科院分区：
文献类型：
--
作者：
Hong J;Kim YK;Won DJ;Kim J;Lee SJ
在这里，我们开发了一个三维DMF（3D DMF）平台与图案化的电极浸没在油介质中，提供基本的解决方案，二维DMF平台和水-空气系统的技术限制。图案化电极上的3D液滴操纵通过可编程地控制电信号来演示。我们还展示了通过不同化学样品的反应在3D DMF平台上形成沉淀物。悬浮在顶部电极上的含有沉淀物的液滴可以在没有沉淀物粘附到固体表面的情况下进行操作。这种方法可以很好地解决二维DMF体系存在的交叉污染和溶质吸附等问题。此外，我们通过引入一个简单的加热过程，确定了在3D DMF平台上进行温度控制化学反应的可行性。为了证明3D DMF系统对3D生物过程的适用性，我们检查了在3D DMF平台中含有小鼠成纤维细胞的液滴的3D操作。最后，我们采用电弹性毛细现象（EEC），展示了柔性薄膜包裹液滴的分离。就业的EEC可能提供一个强大的潜力，在开发的3D DMF平台的药物封装和驱动的微机电设备。
We here develop a three-dimensional DMF (3D DMF) platform with patterned electrodes submerged in an oil medium to provide fundamental solutions to the technical limitations of 2D DMF platforms and water–air systems. 3D droplet manipulation on patterned electrodes is demonstrated by programmably controlling electrical signals. We also demonstrate the formation of precipitates on the 3D DMF platform through the reaction of different chemical samples. A droplet containing precipitates, hanging on the top electrode, can be manipulated without adhesion of precipitates to the solid surface. This method could be a good alternative strategy to alleviate the existing problems of 2D DMF systems such as cross-contamination and solute adsorption. In addition, we ascertain the feasibility of temperature-controlled chemical reaction on the 3D DMF platform by introducing a simple heating process. To demonstrate applicability of the 3D DMF system to 3D biological process, we examine the 3D manipulation of droplets containing mouse fibroblasts in the 3D DMF platform. Finally, we show detachment of droplets wrapped by a flexible thin film by adopting the electro-elasto-capillarity (EEC). The employment of the EEC may offer a strong potential in the development of 3D DMF platforms for drug encapsulation and actuation of microelectromechanical devices.
登录
查看更多内容
影响因子：
2.7
作者：
Cho, SK;Moon, HJ;Kim, CJ
通讯作者：
Kim, CJ
影响因子：
6.1
作者：
Fan, Shih-Kang;Yang, Hanping;Hsu, Wensyang
通讯作者：
Hsu, Wensyang
影响因子：
6.1
作者：
Hong, Jiwoo;Lee, Sang Joon
通讯作者：
Lee, Sang Joon
影响因子：
1.7
作者：
Lee, Seung Jun;Lee, Sanghyun;Kang, Kwan Hyoung
通讯作者：
Kang, Kwan Hyoung
影响因子：
2.8
作者：
Bhattacharjee, Biddut;Najjaran, Homayoun
通讯作者：
Najjaran, Homayoun