Owl Monkey Alu Insertion Polymorphisms and Aotus Phylogenetics.
Owl Monkey Alu Insertion Polymorphisms and Aotus Phylogenetics.
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DOI：
10.3390/genes13112069
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发表时间：
2022-11-08
期刊：
影响因子：
3.5
通讯作者：
Batzer, Mark A. A.
中科院分区：
文献类型：
--
作者：
Storer, Jessica M. M.;Walker, Jerilyn A. A.;Rewerts, Lydia C. C.;Brown, Morgan A. A.;Beckstrom, Thomas O. O.;Herke, Scott W. W.;Roos, Christian;Batzer, Mark A. A.
夜猴（学名：Owl monkeys），又称夜猴，是一种长鼻猴科（Aotidae）的灵长类动物。早期的分类学只承认一个物种，Aotus trivirgatus，直到1983年，Hershkovitz提出了九个独特的物种名称，主要根据皮毛颜色分为红颈和灰颈物种组。最近的研究质疑了这种传统的属的分离，并提出了基于野生种群地理位置的命名。Alu反转录转座子是一类移动的插入元件（MEI），广泛应用于灵长类动物的遗传学研究. Aotus nancymaae [Anan_2.0]的一种Aotus物种的支架水平基因组组装促进了近2000个年轻谱系特异性Alu插入的大规模确定。这项研究提供了候选寡核苷酸位点特异性PCR检测超过1350这些元素。对于四个分类群和多个样本的314个Alu元件，PCR分析鉴定出159个插入多态性，包括21个A组。nancymaae和Aotus azarae（红颈种）为姐妹类群，Aotus vociferans和A. trivirgatus（灰颈）更基部。DNA测序确定了五个新的Alu元素从三个不同的类群。本研究中报告的Alu数据集将有助于物种鉴定，并为野生种群的遗传学，种群遗传学和保护策略提供有价值的资源。
Owl monkeys (genus Aotus), or “night monkeys” are platyrrhine primates in the Aotidae family. Early taxonomy only recognized one species, Aotus trivirgatus, until 1983, when Hershkovitz proposed nine unique species designations, classified into red-necked and gray-necked species groups based predominately on pelage coloration. Recent studies questioned this conventional separation of the genus and proposed designations based on the geographical location of wild populations. Alu retrotransposons are a class of mobile element insertion (MEI) widely used to study primate phylogenetics. A scaffold-level genome assembly for one Aotus species, Aotus nancymaae [Anan_2.0], facilitated large-scale ascertainment of nearly 2000 young lineage-specific Alu insertions. This study provides candidate oligonucleotides for locus-specific PCR assays for over 1350 of these elements. For 314 Alu elements across four taxa with multiple specimens, PCR analyses identified 159 insertion polymorphisms, including 21 grouping A. nancymaae and Aotus azarae (red-necked species) as sister taxa, with Aotus vociferans and A. trivirgatus (gray-necked) being more basal. DNA sequencing identified five novel Alu elements from three different taxa. The Alu datasets reported in this study will assist in species identification and provide a valuable resource for Aotus phylogenetics, population genetics and conservation strategies when applied to wild populations.
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