PeaTAR1B: Characterization of a Second Type 1 Tyramine Receptor of the American Cockroach, Periplaneta americana.
PeaTAR1B: Characterization of a Second Type 1 Tyramine Receptor of the American Cockroach, Periplaneta americana.
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DOI：
10.3390/ijms18112279
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发表时间：
2017-10-30
影响因子：
5.6
通讯作者：
Baumann A
中科院分区：
文献类型：
--
作者：
Blenau W;Balfanz S;Baumann A
儿茶酚胺去甲肾上腺素和肾上腺素调节脊椎动物重要的生理功能。在昆虫;这些神经活性物质在功能上被酚胺类物质章鱼胺和酪胺所取代。酚胺激活特异性鸟嘌呤核苷酸结合(G)蛋白偶联受体（gpcr）。1型酪胺受体被酪胺比章鱼胺更好地激活。相比之下;2型酪胺受体几乎完全由酪胺激活。功能;1型酪胺受体的激活导致细胞内cAMP （[cAMP]i）浓度的降低，而2型酪胺受体可以介导Ca2+信号或Ca2+信号和对[cAMP]i的影响。在这里;我们报道美洲大蠊（Periplaneta americana）除了表达PeaTAR1A（以前称为PeaTYR1）外，还表达另一种1型酪胺受体（PeaTAR1B）。在flpTM细胞中异种表达时；酪胺激活PeaTAR1B导致[cAMP]i的浓度依赖性降低。它的活性可被一系列已建立的拮抗剂阻断。美洲两种1型酪胺受体的功能特性PeaTAR1A和PeaTAR1B；通过改变cAMP水平对酪胺做出反应；是了解酪胺在蟑螂生理和行为中的作用的重要一步；尤其是与章鱼胺的作用相比。
The catecholamines norepinephrine and epinephrine regulate important physiological functions in vertebrates. In insects; these neuroactive substances are functionally replaced by the phenolamines octopamine and tyramine. Phenolamines activate specific guanine nucleotide-binding (G) protein-coupled receptors (GPCRs). Type 1 tyramine receptors are better activated by tyramine than by octopamine. In contrast; type 2 tyramine receptors are almost exclusively activated by tyramine. Functionally; activation of type 1 tyramine receptors leads to a decrease in the intracellular concentration of cAMP ([cAMP]i) whereas type 2 tyramine receptors can mediate Ca2+ signals or both Ca2+ signals and effects on [cAMP]i. Here; we report that the American cockroach (Periplaneta americana) expresses a second type 1 tyramine receptor (PeaTAR1B) in addition to PeaTAR1A (previously called PeaTYR1). When heterologously expressed in flpTM cells; activation of PeaTAR1B by tyramine leads to a concentration-dependent decrease in [cAMP]i. Its activity can be blocked by a series of established antagonists. The functional characterization of two type 1 tyramine receptors from P. americana; PeaTAR1A and PeaTAR1B; which respond to tyramine by changing cAMP levels; is a major step towards understanding the actions of tyramine in cockroach physiology and behavior; particularly in comparison to the effects of octopamine.
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影响因子：
9.2
作者：
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通讯作者：
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影响因子：
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通讯作者：
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通讯作者：
Baumann A
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通讯作者：
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