Engineering the Bacterial Microcompartment Domain for Molecular Scaffolding Applications
Engineering the Bacterial Microcompartment Domain for Molecular Scaffolding Applications
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为分子支架应用设计细菌微区室域
DOI：
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发表时间：
2017
影响因子：
5.2
通讯作者：
Daniel C. Ducat
中科院分区：
文献类型：
--
作者：
E. Young;R. Burton;J. Mahalik;B. Sumpter;M. Fuentes;C. Kerfeld;Daniel C. Ducat
随着合成生物学推进工程生物系统的复杂性，细胞环境中空间组织的重要性决不能被边缘化。生物工程师越来越多地研究控制细胞内空间组织的方法，模仿自然途径用来增加通量和减少串扰的策略。用于构建多种定义的可编程架构的模块化平台将极大地有助于提高引入的代谢途径的产量并增加其他异源系统的隔离。在这里，我们回顾了细菌微区室外壳蛋白的最新研究，并讨论了它们作为一系列定制细胞内支架的“构建块”的潜在应用。我们总结了 BMC 壳蛋白自组装的知识现状，并讨论了未来的研究途径，这对于实现 BMC 壳蛋白作为生物工程的预测性组装和可编程生物材料的潜力非常重要。
As synthetic biology advances the intricacy of engineered biological systems, the importance of spatial organization within the cellular environment must not be marginalized. Increasingly, biological engineers are investigating means to control spatial organization within the cell, mimicking strategies used by natural pathways to increase flux and reduce cross-talk. A modular platform for constructing a diverse set of defined, programmable architectures would greatly assist in improving yields from introduced metabolic pathways and increasing insulation of other heterologous systems. Here, we review recent research on the shell proteins of bacterial microcompartments and discuss their potential application as “building blocks” for a range of customized intracellular scaffolds. We summarize the state of knowledge on the self-assembly of BMC shell proteins and discuss future avenues of research that will be important to realize the potential of BMC shell proteins as predictively assembling and programmable biological materials for bioengineering.
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影响因子：
12.4
作者：
Bashor CJ;Horwitz AA;Peisajovich SG;Lim WA
通讯作者：
Lim WA
细菌微型室内细胞器：蛋白质壳的结构和进化。
影响因子：
12.4
作者：
Yeates TO;Crowley CS;Tanaka S
通讯作者：
Tanaka S
影响因子：
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作者：
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通讯作者：
Bobik, Thomas A.
影响因子：
15
作者：
Fu, Jinglin;Liu, Minghui;Liu, Yan;Woodbury, Neal W.;Yan, Hao
通讯作者：
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影响因子：
38.3
作者：
Funke, Jonas J.;Dietz, Hendrik
通讯作者：
Dietz, Hendrik