Immune Evaluation of Recombinant Lactobacillus plantarum With Surface Display of HA1-DCpep in Mice.
Immune Evaluation of Recombinant Lactobacillus plantarum With Surface Display of HA1-DCpep in Mice.
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DOI：
10.3389/fimmu.2021.800965
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发表时间：
2021
影响因子：
7.3
通讯作者：
Wang CF
中科院分区：
文献类型：
--
作者：
Niu H;Xing JH;Zou BS;Shi CW;Huang HB;Jiang YL;Wang JZ;Cao X;Wang N;Zeng Y;Yang WT;Yang GL;Wang CF
禽流感病毒可以通过粘膜有效传播，传统疫苗无法有效预防流感病毒的粘膜感染。为了在生物体中诱导多重免疫应答，我们构建了表达流感病毒抗原HA 1的重组植物乳杆菌，其具有佐剂树突状细胞靶向肽（DCpep）。重组L.以植物根瘤菌NC 8 Δ-pWCF-HA 1和NC 8 Δ-pWCF-HA 1-DCpep经口免疫小鼠，观察小鼠的体液、细胞和粘膜免疫应答。此外，还测定了血清特异性抗体水平和血凝抑制（HI）水平。结果表明，重组植物乳杆菌可激活派尔集合淋巴结（Peyer's patches，PP s）中的树突状细胞，增加脾脏和肠系膜淋巴结（MLNs）中CD 4+和CD 8 +IFN-γ+细胞的数量，并影响脾脏和MLNs中CD 4+和CD 8+细胞的增殖能力。此外，重组L.结果表明，植物提取物能增加PP中B220+伊加+细胞的数量，并能增加肺和不同肠段中伊加的含量。此外，在小鼠血清中诱导高水平的特异性IgG、IgG 1和IgG 2a抗体，在小鼠粪便中诱导高水平的特异性伊加抗体，HI效价显著增加。因此，重组L.植物株NC 8 Δ-pWCF-HA 1和NC 8 Δ-pWCF-HA 1-DCpep具有作为禽流感病毒候选疫苗的潜力。
Avian influenza viruses can be efficiently transmitted through mucous membranes, and conventional vaccines are not effective in protecting against mucosal infection by influenza viruses. To induce multiple immune responses in an organism, we constructed a recombinant Lactobacillus plantarum expressing the influenza virus antigen HA1 with the adjuvant dendritic cell-targeting peptide (DCpep). The recombinant L. plantarum strains NC8Δ-pWCF-HA1 and NC8Δ-pWCF-HA1-DCpep were used to immunize mice via oral administration, and the humoral, cellular and mucosal immune responses were evaluated. In addition, the serum levels of specific antibodies and hemagglutination inhibition (HI) levels were also measured. Our results showed that recombinant L. plantarum activated dendritic cells in Peyer’s patches (PPs), increased the numbers of CD4+IFN-γ+ and CD8+IFN-γ+ cells in the spleen and mesenteric lymph nodes (MLNs), and affected the ability of CD4+ and CD8+ cells to proliferate in the spleen and MLNs. Additionally, recombinant L. plantarum increased the number of B220+IgA+ cells in PPs and the level of IgA in the lungs and different intestinal segments. In addition, specific IgG, IgG1 and IgG2a antibodies were induced at high levels in the mice serum, specific IgA antibodies were induced at high levels in the mice feces, and HI potency was significantly increased. Thus, the recombinant L. plantarum strains NC8Δ-pWCF-HA1 and NC8Δ-pWCF-HA1-DCpep have potential as vaccine candidates for avian influenza virus.
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影响因子：
3.7
作者：
Park, Min-Kyung;Vu Ngo;Kang, Sang-Moo
通讯作者：
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影响因子：
2.8
作者：
Hajam, Irshad Ahmed;Kirthika, Perumalraja;Lee, John Hwa
通讯作者：
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表达血凝素的植物乳杆菌疫苗载体可提供针对 H9N2 攻击感染的保护
影响因子：
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作者：
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通讯作者：
Wang, Chun-Feng
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影响因子：
4.1
作者：
Jiang, Yanlong;Yang, Guilian;Wang, Chunfeng
通讯作者：
Wang, Chunfeng
影响因子：
4.4
作者：
Kamble, Nitin Machindra;Hyoung, Kim Je;Lee, John Hwa
通讯作者：
Lee, John Hwa