Positive Feedback Keeps Duration of Mitosis Temporally Insulated from Upstream Cell-Cycle Events.
Positive Feedback Keeps Duration of Mitosis Temporally Insulated from Upstream Cell-Cycle Events.
复制标题
DOI：
10.1016/j.molcel.2016.09.018
复制
发表时间：
2016-10-20
期刊：
影响因子：
16
通讯作者：
Santos, Silvia D. M.
中科院分区：
文献类型：
--
作者：
Araujo, Ana Rita;Gelens, Lendert;Sheriff, Rahuman S. M.;Santos, Silvia D. M.
细胞分裂的特征是一个细胞产生两个子细胞的一系列事件。单细胞周期动力学的定量测量表明，尽管G1期、S期和G2期存在差异，但有丝分裂的持续时间很短，而且非常恒定。令人惊讶的是，细胞周期长度和有丝分裂持续时间之间没有相关性，这表明有丝分裂在时间上与细胞周期早期阶段的可变性隔绝。通过结合活细胞成像和计算模型，我们表明正反馈是有丝分裂暂时隔离的分子机制。扰动正反馈导致通过有丝分裂的缓慢、可变的进入和进展，以及有丝分裂的持续时间与细胞周期长度的可变性无关。我们表明，正反馈对于保持有丝分裂的短、恒定和时间隔离是重要的，并预计这可能是在其他生物系统中创建模块化的一种常用调节策略。单细胞有丝分裂的持续时间很短，而且非常恒定，有丝分裂的持续时间与细胞周期早期的可变性在时间上是隔绝的，正反馈使有丝分裂持续时间短、恒定，隔离的正反馈可以是一种细胞策略，在信号传递中实现模块化。通过定量的单细胞测量表明，有丝分裂是短暂的，相当恒定的，令人惊讶的是，与早期细胞周期阶段高度可变的持续时间无关。他们提出，正反馈是这种模块化的原因，并预测它可能会在其他生物系统中实现模块化。
Cell division is characterized by a sequence of events by which a cell gives rise to two daughter cells. Quantitative measurements of cell-cycle dynamics in single cells showed that despite variability in G1-, S-, and G2 phases, duration of mitosis is short and remarkably constant. Surprisingly, there is no correlation between cell-cycle length and mitotic duration, suggesting that mitosis is temporally insulated from variability in earlier cell-cycle phases. By combining live cell imaging and computational modeling, we showed that positive feedback is the molecular mechanism underlying the temporal insulation of mitosis. Perturbing positive feedback gave rise to a sluggish, variable entry and progression through mitosis and uncoupled duration of mitosis from variability in cell cycle length. We show that positive feedback is important to keep mitosis short, constant, and temporally insulated and anticipate it might be a commonly used regulatory strategy to create modularity in other biological systems. Duration of mitosis in single cells is short and remarkably constant Duration of mitosis is temporally insulated from variability in early cell-cycle phases Positive feedback keeps duration of mitosis short, constant, and insulated Positive feedback can be a cellular strategy to bring about modularity in signaling Araujo et al. show by quantitative single cell measurements that mitosis is short, fairly constant, and, surprisingly, uncoupled from the highly variable duration of earlier cell-cycle phases. They propose that positive feedback is responsible for this modularity and predict that it might enable modularity in other biological systems.
登录
查看更多内容
影响因子：
64.5
作者：
Bouchoux, Celine;Uhlmann, Frank
通讯作者：
Uhlmann, Frank
DOI：
10.1073/pnas.94.6.2168
发表时间：
1997-03-18
影响因子：
11.1
作者：
Kwon, YG;Lee, SY;Nairn, AC
通讯作者：
Nairn, AC
影响因子：
64.8
作者：
Hartwell, LH;Hopfield, JJ;Murray, AW
通讯作者：
Murray, AW
影响因子：
64.5
作者：
LI, R;MURRAY, AW
通讯作者：
MURRAY, AW
影响因子：
64.8
作者：
MURRAY, AW;KIRSCHNER, MW
通讯作者：
KIRSCHNER, MW