SpoVID functions as a non-competitive hub that connects the modules for assembly of the inner and outer spore coat layers in Bacillus subtilis.
SpoVID functions as a non-competitive hub that connects the modules for assembly of the inner and outer spore coat layers in Bacillus subtilis.
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DOI：
10.1111/mmi.14116
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发表时间：
2018-11
影响因子：
3.6
通讯作者：
Henriques AO
中科院分区：
文献类型：
--
作者：
Nunes F;Fernandes C;Freitas C;Marini E;Serrano M;Moran CP Jr;Eichenberger P;Henriques AO
在枯草芽孢杆菌的产孢过程中，一组母细胞特异性蛋白质指导被毛的组装，这是一种保护孢子并影响其许多环境相互作用的多蛋白结构。SafA和CotE充当聚会中心，管理内层和外层涂层的组装。将外壳蛋白靶向发育中的孢子，然后进行包衣。SafA和CotE的包裹需要E，这是包裹蛋白SpoVID中包含11个氨基酸的区域，CotE与E直接相互作用。在这里，我们在E中发现了两个单丙氨酸取代，它们阻止了SafA与SpoVID的结合，而不是CotE与SpoVID的结合，并阻断了包膜。替换导致SafA、CotE及其依赖蛋白在母细胞近端孢子极积累，表型复制了一个spoVID零突变体，并表明错定位的SafA对外壳的其余部分起吸引作用。在SafA结合中对E的要求被一个反式提供E序列的肽所绕过。我们认为E允许SafA结合到SpoVID的第二个区域，使CotE与E和SpoVID相互作用，在孢子包裹过程中充当非竞争性枢纽。
During sporulation in Bacillus subtilis, a group of mother cell‐specific proteins guides the assembly of the coat, a multiprotein structure that protects the spore and influences many of its environmental interactions. SafA and CotE behave as party hubs, governing assembly of the inner and outer coat layers. Targeting of coat proteins to the developing spore is followed by encasement. Encasement by SafA and CotE requires E, a region of 11 amino acids in the encasement protein SpoVID, with which CotE interacts directly. Here, we identified two single alanine substitutions in E that prevent binding of SafA, but not of CotE, to SpoVID, and block encasement. The substitutions result in the accumulation of SafA, CotE and their dependent proteins at the mother cell proximal spore pole, phenocopying a spoVID null mutant and suggesting that mislocalized SafA acts as an attractor for the rest of the coat. The requirement for E in SafA binding is bypassed by a peptide with the sequence of E provided in trans. We suggest that E allows binding of SafA to a second region in SpoVID, enabling CotE to interact with E and SpoVID to function as a non‐competitive hub during spore encasement.
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