Homer2 within the central nucleus of the amygdala modulates withdrawal-induced anxiety in a mouse model of binge-drinking.
Homer2 within the central nucleus of the amygdala modulates withdrawal-induced anxiety in a mouse model of binge-drinking.
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DOI：
10.1016/j.neuropharm.2017.11.001
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发表时间：
2018-01
影响因子：
4.7
通讯作者：
Szumlinski KK
中科院分区：
文献类型：
--
作者：
Lee KM;Coelho MA;Sern KR;Szumlinski KK
A history of binge-drinking decreases protein expression of the glutamate-related scaffolding protein Homer2 within the central nucleus of the amygdala (CEA), coinciding with behavioral signs of negative affect. To assess the functional relevance of this protein change for withdrawal-induced hyper-anxiety, adult (PND 56) and adolescent (PND 28) male C57BL/6J mice were administered an intra-CEA infusion of an adeno-associated viral vector (AAV) carrying either cDNA to express Homer2 (H2-cDNA) or GFP as control. Mice underwent 14 days of binge-drinking under multi-bottle, limited-access conditions and were assayed for behavioral signs of negative affect during withdrawal using the light-dark box, marble burying, and forced swim tests (FST). Following behavioral testing, all animals experienced 5 days of drinking to evaluate the effects of prior alcohol experience and Homer2 manipulation on subsequent alcohol consumption. During protracted (4 weeks) withdrawal, adolescent alcohol-experienced GFP controls showed increased signs of negative affect across all 3 assays, compared to water-drinking GFP animals, and also showed elevated alcohol consumption during the subsequent drinking period. Homer2-cDNA infusion in adolescent-onset alcohol-drinking animals was anxiolytic and reduced subsequent alcohol consumption. Conversely, Homer2-cDNA was anxiogenic and increased drinking in water-drinking adolescents. Unfortunately, the data from adult-onset alcohol-drinking animals were confounded by low alcohol consumption and negligible behavioral signs of anxiety. Nevertheless, the present results provide novel cause-effect evidence supporting a role for CEA Homer2 in the regulation of both basal anxiety and the time-dependent intensification of negative affective states in individuals with a history of binge-drinking during adolescence.
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DOI：
10.1111/j.1601-183x.2010.00647.x
发表时间：
2011-02
期刊：
Genes, brain, and behavior
影响因子：
--
作者：
Goulding SP;Obara I;Lominac KD;Gould AT;Miller BW;Klugmann M;Szumlinski KK
通讯作者：
Szumlinski KK
影响因子：
--
作者：
Beesdo, Katja;Lau, Jennifer Y. F.;Guyer, Amanda E.;McClure-Tone, Erin B.;Monk, Christopher S.;Nelson, Eric E.;Fromm, Stephen J.;Goldwin, Michelle A.;Wittchen, Hans-Ulrich;Leibenluft, Ellen;Erns, Monique;Pine, Daniel S.
通讯作者：
Pine, Daniel S.
影响因子：
10.6
作者：
Davidson, RJ
通讯作者：
Davidson, RJ
影响因子：
5.5
作者：
Esser MB;Hedden SL;Kanny D;Brewer RD;Gfroerer JC;Naimi TS
通讯作者：
Naimi TS
影响因子：
64.8
作者：
Brakeman, PR;Lanahan, AA;Worley, PF
通讯作者：
Worley, PF