Circulating miR-30b-5p levels in plasma as a novel potential biomarker for early detection of breast cancer.
Circulating miR-30b-5p levels in plasma as a novel potential biomarker for early detection of breast cancer.
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血浆中循环miR-30b-5p水平作为早期检测乳腺癌的新型潜在生物标志物
DOI：
10.1016/j.esmoop.2020.100039
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发表时间：
2021-03
期刊：
影响因子：
7.3
通讯作者：
Cejalvo JM
中科院分区：
文献类型：
--
作者：
Adam-Artigues A;Garrido-Cano I;Simón S;Ortega B;Moragón S;Lameirinhas A;Constâncio V;Salta S;Burgués O;Bermejo B;Henrique R;Lluch A;Jerónimo C;Eroles P;Cejalvo JM
最近，microRNAs已被证明是一种潜在的非侵入性生物标志物，用于癌症的诊断、预后评估或治疗反应的预测。在本研究中，我们评价miR-30b-5p作为组织和血浆中乳腺癌(BC)早期诊断生物标志物的潜力。检测了112例乳腺癌和40例正常乳腺组织中miR-30b-5p的表达。在38名BC患者和40名健康献血者的发现队列中，以及83名BC患者和83名健康志愿者的验证队列中，检测了血浆样本中循环miR-30b-5p的水平。用实时定量聚合酶链式反应检测miR-30b-5p的表达，并进行受试者工作特征曲线分析。乳腺癌组织中miR-30b-5p的表达明显低于正常乳腺组织。相比之下，BC患者的循环miR-30b-5p水平显著高于健康献血者。此外，腋窝淋巴结阳性和新发转移患者外周血中miR-30b-5p水平显著升高。受试者工作特性曲线分析表明，miR-30b-5p在早期即可检出BC。因此，我们强调miR-30b-5p作为一种无创、快速、可重复性和经济有效的BC诊断生物标志物的潜力。结果表明：与正常乳腺组织相比，乳腺癌组织中miR-30b-5p的表达较低。血浆中高水平的miR-30b-5p与乳腺癌的发生有关。血浆中的MIR-30b-5p水平可识别所有亚型和疾病阶段的乳腺癌。循环miR-30b-5p水平与患者的肿瘤负荷有关。循环中的miR-30b-5p是一种潜在的无创、经济有效的诊断BC的生物标志物。
Recently, microRNAs have been demonstrated to be potential non-invasive biomarkers for diagnosis, prognosis assessment or prediction of response to treatment in cancer. In this study, we evaluate the potential of miR-30b-5p as a biomarker for early diagnosis of breast cancer (BC) in tissue and plasma. Expression of miR-30b-5p was determined in a series of 112 BC and 40 normal breast tissues. Circulating miR-30b-5p levels in plasma samples were determined in a discovery cohort of 38 BC patients and 40 healthy donors and in a validation cohort of 83 BC patients and 83 healthy volunteers. miR-30b-5p expression was measured by quantitative real-time PCR and receiver operating characteristics curve analysis was carried out. The miR-30b-5p expression was significantly lower in BC tissue than in healthy breast samples. In contrast, circulating miR-30b-5p levels were significantly higher in BC patients compared with healthy donors. Furthermore, circulating miR-30b-5p levels were significantly higher in patients with positive axillary lymph node and de novo metastatic patients. Receiver operating characteristics curve analysis demonstrated a good diagnostic potential of miR-30b-5p to detect BC even at an early stage of the disease. Thus, we highlight the potential of miR-30b-5p as a non-invasive, fast, reproducible and cost-effective diagnostic biomarker of BC. Our results show the following: Low miR-30b-5p expression was detected in breast tumor tissue compared with healthy breast tissue. High circulating miR-30b-5p levels in plasma were associated to breast cancer. miR-30b-5p levels in plasma identify breast cancer of all subtypes and stages of the disease. Circulating miR-30b-5p levels relate with patients' tumor burden. Circulating miR-30b-5p is a potential non-invasive and cost-effective diagnostic BC biomarker.
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